Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDKL6 – Sulfite reductase flavoprotein-60 reduced with 10 molar equivalents sodium dithionite

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data

Sample:	Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11

Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M

R_g^Guinier	3.4	nm
D_max	12.2	nm

SASDKM6 – Sulfite reductase flavoprotein-60 (contrast-matched)/deuterated-hemoprotein heterodimer

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) experimental SAS data

Sample:	Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 Jun 28

Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M

R_g^Guinier	2.4	nm
D_max	6.6	nm

SASDKN6 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein (contrast-matched) heterodimer

Sample:	Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 Jun 26

Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M

R_g^Guinier	3.2	nm
D_max	12.4	nm

SASDL62 – The truncated RTX domain of adenylate cyclase toxin CyaA - construct RTX-1 (amino acids 1132-1294 and 1562-1681 of CyaA)

hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) experimental SAS data

Sample:	Hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) monomer, 30 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

Almost half of the RTX domain is dispensable for complement receptor 3 binding and cell-invasive activity of the adenylate cyclase toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L

R_g^Guinier	2.4	nm
D_max	8.2	nm
Volume^Porod	41	nm³

SASDL72 – The truncated RTX domain of adenylate cyclase toxin CyaA - construct RTX-2 (amino acids 1132-1303 and 1562-1681 of CyaA)

hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) experimental SAS data

Sample:	Hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) monomer, 31 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	37	nm³

SASDK58 – Histidine kinase AdeS - cytoplasmic domain

Sample:	Histidine kinase hexamer, 186 kDa Acinetobacter baumannii protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 19

Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience 24(5):102476 (2021)
Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y

R_g^Guinier	4.6	nm
D_max	16.3	nm
Volume^Porod	378	nm³

SASDK24 – Signal recognition particle SRP9/14 heterodimer from Plasmodium falciparum

Signal recognition particle 9Signal recognition particle 14 experimental SAS data

Sample:	Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 22

Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation. Commun Biol 4(1):600 (2021)
Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I

R_g^Guinier	2.1	nm
D_max	7.2	nm
Volume^Porod	47	nm³

SASDK34 – Full length SRP Alu RNA from Plasmodium falciparum

Full-length SRP Alu RNA experimental SAS data

Sample:	Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.3	nm
D_max	11.8	nm
Volume^Porod	63	nm³

SASDK44 – The 5' domain of SRP Alu RNA from Plasmodium falciparum

SRP Alu RNA 5' domain experimental SAS data

Sample:	SRP Alu RNA 5' domain monomer, 24 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	38	nm³

SASDK54 – Signal recognition particle SRP9/14 heterodimer in complex with full length SRP Alu RNA from Plasmodium falciparum

Signal recognition particle 9Signal recognition particle 14Full-length SRP Alu RNA experimental SAS data

Sample:	Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	120	nm³

First << Prev Page 42 of 92 Next >> Last