Browse by MODEL: Ab initio only

SASDJS4 – Type 3 secretion system pilotin, InvH70-147

Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods. Structure (2020)
Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 29 nm3

SASDJC6 – Human Vitamin K-dependent protein C

Vitamin K-dependent protein C experimental SAS data
DAMFILT model
Sample: Vitamin K-dependent protein C monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 145 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 14
Zymogen and activated protein C have similar structural architecture. J Biol Chem (2020)
Stojanovski BM, Pelc LA, Zuo X, Di Cera E
RgGuinier 3.6 nm
Dmax 14.0 nm

SASDJD6 – Human Vitamin K-dependent Activated Protein C

Vitamin K-dependent protein C experimental SAS data
DAMFILT model
Sample: Vitamin K-dependent protein C monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 145 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Aug 17
Zymogen and activated protein C have similar structural architecture. J Biol Chem (2020)
Stojanovski BM, Pelc LA, Zuo X, Di Cera E
RgGuinier 3.7 nm
Dmax 14.0 nm

SASDJS3 – Adenylate cyclase toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 94 nm3

SASDJT3 – Adenylate cyclase toxin CyaA RTX block III-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 3.4 nm
Dmax 12.1 nm
VolumePorod 85 nm3

SASDJU3 – Adenylate cyclase toxin CyaA RTX block IV-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 45 nm3

SASDHU9 – Solution structure of middle domain of the multi-domain GH18 chitinase ChiA from Flavobacterium johnsoniae

Chitinase ChiA experimental SAS data
DAMMIN model
Sample: Chitinase ChiA monomer, 70 kDa Flavobacterium johnsoniae protein
Buffer: 50 mM Tris, 250 mM NaCl, 0.25 mM DTT, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 May 7
Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae. Sci Rep 10(1):13775 (2020)
Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Brändén G, Larsbrink J
RgGuinier 4.7 nm
Dmax 16.3 nm
VolumePorod 99 nm3

SASDJE2 – Beta-ketoacyl synthase (Bamb_5925) C-terminal docking domain

Beta-ketoacyl synthase Bamb_5925 experimental SAS data
DAMMIN model
Sample: Beta-ketoacyl synthase Bamb_5925 monomer, 4 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 3 nm3

SASDJG2 – Beta-ketoacyl synthase (Bamb_5920) C-terminal docking domain

Beta-ketoacyl synthase Bamb_5920 experimental SAS data
DAMMIN model
Sample: Beta-ketoacyl synthase Bamb_5920 monomer, 2 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.2 nm
Dmax 4.9 nm
VolumePorod 3 nm3

SASDHD9 – Full-length Plasmodium falciparum Heat shock protein 90 (PfHsp90)

Plasmodium falciparum Heat shock protein 90 experimental SAS data
DAMMIN model
Sample: Plasmodium falciparum Heat shock protein 90 dimer, 177 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
Silva N, Torricillas M, Minari K, Barbosa L, Seraphim T, Borges J
RgGuinier 5.7 nm
Dmax 19.0 nm
VolumePorod 350 nm3