Browse by MODEL: Ab initio only

SASDHU5 – Heat shock protein 70 (PfHSP70-1)

Heat shock protein 70Peptide experimental SAS data
DAMFILT model
Sample: Heat shock protein 70 monomer, 74 kDa Plasmodium falciparum protein
Peptide monomer, 1 kDa synthetic construct protein
Buffer: 50mM Tris, 5mM MgCl2, 500mM KCl, 5mM Bme, 5% glycerol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24
Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
Anas M, Shukla A, Tripathi A, Kumari V, Prakash C, Nag P, Kumar LS, Sharma SK, Ramachandran R, Kumar N
RgGuinier 5.3 nm
Dmax 16.6 nm
VolumePorod 450 nm3

SASDJW9 – Mammalian cell entry protein 4A (Mce4A36-400)

Mce-family protein Mce4An-Dodecyl-β-D-Maltopyranoside experimental SAS data
OTHER model
Sample: Mce-family protein Mce4A monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 13
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 5.7 nm
Dmax 21.5 nm
VolumePorod 446 nm3

SASDJX9 – Mammalian cell entry protein 4A (Mce4A121-400)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data
CUSTOM IN-HOUSE model
Sample: N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 35 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Nov 28
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 5.0 nm
Dmax 19.1 nm
VolumePorod 278 nm3

SASDJY9 – Mammalian cell entry protein 4A (Mce4A39-320)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data
CUSTOM IN-HOUSE model
Sample: N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 2
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 4.8 nm
Dmax 18.2 nm
VolumePorod 189 nm3

SASDJZ9 – Mammalian cell entry protein 1A (Mce1A38-325)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 36 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 82 kDa
Buffer: 50 mM Tris, 500 mM NaCl, 10% Glycerol, 5 mM DDM, 1 mM β-ME, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 2
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 4.6 nm
Dmax 17.5 nm
VolumePorod 151 nm3

SASDK22 – Mammalian cell entry protein 1A (Mce1A126-454)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
Buffer: 50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Nov 28
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 5.5 nm
Dmax 22.0 nm
VolumePorod 216 nm3

SASDK32 – Mammalian cell entry protein 1A (Mce1A38-454)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 48 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 112 kDa
Buffer: 50 mM Tris, 500 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 13
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 5.3 nm
Dmax 21.6 nm
VolumePorod 362 nm3

SASDC72 – Alcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrogenase (P0A9Q8)

Aldehyde-alcohol dehydrogenase experimental SAS data
DAMMIF model
Sample: Aldehyde-alcohol dehydrogenase dimer, 97 kDa Escherichia coli O157:H7 protein
Buffer: 20 mM Tris 400 mM NaCl 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Nov 27
High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE. Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020)
Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M
RgGuinier 3.2 nm
Dmax 16.9 nm
VolumePorod 146 nm3

SASDCK3 – Aldehyde dehydrogenase

Aldehyde-alcohol dehydrogenase experimental SAS data
DAMMIF model
Sample: Aldehyde-alcohol dehydrogenase monomer, 48 kDa Escherichia coli O157:H7 protein
Buffer: 30 mM HEPES, 150 mM NaCl, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 30
High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE. Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020)
Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M
RgGuinier 2.7 nm
Dmax 11.1 nm
VolumePorod 74 nm3

SASDJR4 – Type 3 secretion system pilotin, InvH27-147

Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 28 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods. Structure (2020)
Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ
RgGuinier 3.1 nm
Dmax 12.6 nm
VolumePorod 53 nm3