Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDDM5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA) with KCl

octo-repeat PrP mRNA mutant experimental SAS data

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM KCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z

R_g^Guinier	9.0	nm
D_max	33.0	nm
Volume^Porod	210	nm³

SASDDN5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with LiCl

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM LiCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	8.9	nm
D_max	33.0	nm
Volume^Porod	223	nm³

SASDDP5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with KCl and pyridostatin (PDS)

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	9.2	nm
D_max	33.0	nm
Volume^Porod	260	nm³

SASDDQ4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) of Insulin gene enhancer protein ISL-2

LIM/homeobox protein Lhx4Insulin gene enhancer protein ISL-2 experimental SAS data

Sample:	LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein Insulin gene enhancer protein ISL-2 monomer, 4 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 19

Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins (2019)
Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM

R_g^Guinier	2.2	nm
D_max	8.0	nm
Volume^Porod	20	nm³

SASDDR4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) R282G mutant of Insulin gene enhancer protein ISL-2

LIM/homeobox protein Lhx4Insulin gene enhancer protein ISL-2 (R282G) experimental SAS data

Sample:	LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein Insulin gene enhancer protein ISL-2 (R282G) monomer, 4 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 19

Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins (2019)
Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM

R_g^Guinier	2.3	nm
D_max	8.5	nm
Volume^Porod	21	nm³

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data

Sample:	Circularized Membrane scaffolding protein 1 E3 D1, 62 kDa protein 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer:	20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 5

Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution The FEBS Journal 286(9):1734-1751 (2019)
Johansen N, Tidemand F, Nguyen T, Rand K, Pedersen M, Arleth L

R_g^Guinier	5.8	nm
D_max	14.5	nm

SASDKM7 – Sulfite reductase flavoprotein-60

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data

Sample:	Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11

NADPH-dependent sulfite reductase flavoprotein adopts an extended conformation unique to this diflavin reductase Journal of Structural Biology 205(2):170-179 (2019)
Tavolieri A, Murray D, Askenasy I, Pennington J, McGarry L, Stanley C, Stroupe M

R_g^Guinier	3.2	nm
D_max	11.6	nm
Volume^Porod	60	nm³

SASDKN7 – Sulfite reductase flavoprotein-60-ΔAAPSQS

Sample:	Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer:	50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment:	SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11

R_g^Guinier	3.2	nm
D_max	11.3	nm
Volume^Porod	73	nm³

SASDE78 – Solution structure of the diadenylate cyclase/phosphoglucosamine mutase (DacA/GlmM) complex from Staphylococcus aureus

Diadenylate cyclasePhosphoglucosamine mutase experimental SAS data

Sample:	Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus protein Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 7

Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM. PLoS Pathog 15(1):e1007537 (2019)
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A

R_g^Guinier	3.9	nm
D_max	12.1	nm
Volume^Porod	204	nm³

SASDE88 – Solution structure of phosphoglucosamine mutase (GlmM) from Staphylococcus aureus

Phosphoglucosamine mutase experimental SAS data

Sample:	Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 7

R_g^Guinier	3.7	nm
D_max	12.5	nm
Volume^Porod	134	nm³

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