Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDGW6 – Serine acetyltransferase

Serine acetyltransferase experimental SAS data

Sample:	Serine acetyltransferase hexamer, 177 kDa Escherichia coli protein
Buffer:	20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH: 7.5
Experiment:	SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2016 Jun 1

Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action. Int J Mol Sci 20(20) (2019)
Rosa B, Marchetti M, Paredi G, Amenitsch H, Franko N, Benoni R, Giabbai B, De Marino MG, Mozzarelli A, Ronda L, Storici P, Campanini B, Bettati S

R_g^Guinier	3.9	nm
D_max	13.0	nm
Volume^Porod	280	nm³

SASDGX6 – Cysteine synthase/Serine acetyltransferase complex

Cysteine synthase A (4-mer)Serine acetyltransferase (6-mer) experimental SAS data

Sample:	Cysteine synthase A (4-mer) tetramer, 143 kDa Escherichia coli protein Serine acetyltransferase (6-mer) hexamer, 177 kDa Escherichia coli protein
Buffer:	20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH: 7.5
Experiment:	SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2016 Jun 1

R_g^Guinier	6.1	nm
D_max	22.0	nm
Volume^Porod	457	nm³

SASDFA6 – Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296)

Flagella binding tail protein experimental SAS data

Sample:	Flagella binding tail protein monomer, 103 kDa Salmonella virus Chi protein
Buffer:	20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 4

The flagellotropic bacteriophage YSD1 targets Salmonella Typhi with a Chi-like protein tail fibre. Mol Microbiol (2019)
Dunstan RA, Pickard D, Dougan S, Goulding D, Cormie C, Hardy J, Li F, Grinter R, Harcourt K, Yu L, Song J, Schreiber F, Choudhary J, Clare S, Coulibaly F, Strugnell RA, Dougan G, Lithgow T

R_g^Guinier	5.6	nm
D_max	27.4	nm
Volume^Porod	155	nm³

SASDEV9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

Cytohesin-2; ARNO truncation mutant experimental SAS data

Sample:	Cytohesin-2; ARNO truncation mutant monomer, 40 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Nov 25

Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG

R_g^Guinier	2.7	nm
D_max	9.9	nm
Volume^Porod	63	nm³

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Cytohesin-2 ARF nucleotide-binding site opener experimental SAS data

Sample:	Cytohesin-2 ARF nucleotide-binding site opener dimer, 93 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 23

R_g^Guinier	4.8	nm
D_max	19.7	nm
Volume^Porod	145	nm³

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Sample:	Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	194	nm³

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Sample:	Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.3	nm
D_max	25.7	nm
Volume^Porod	168	nm³

SASDG26 – Wild type 4-hydroxy-tetrahydrodipicolinate synthase

4-hydroxy-tetrahydrodipicolinate synthase experimental SAS data

Sample:	4-hydroxy-tetrahydrodipicolinate synthase tetramer, 131 kDa Campylobacter jejuni protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase. J Struct Biol :107409 (2019)
Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ

R_g^Guinier	3.4	nm
D_max	9.0	nm
Volume^Porod	188	nm³

SASDG36 – 4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant)

4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) experimental SAS data

Sample:	4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) dimer, 65 kDa Campylobacter jejuni protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

R_g^Guinier	3.1	nm
D_max	9.5	nm
Volume^Porod	108	nm³

SASDG46 – 4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant)

4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant) experimental SAS data

Sample:	4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant), 33 kDa Campylobacter jejuni protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

R_g^Guinier	3.3	nm
D_max	8.9	nm
Volume^Porod	163	nm³

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