Browse by MODEL: Ab initio only

SASDE98 – Solution structure of Diadenylate cyclase (DacA) from Staphylococcus aureus

Diadenylate cyclase experimental SAS data
DAMFILT model
Sample: Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM. PLoS Pathog 15(1):e1007537 (2019)
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A
RgGuinier 2.6 nm
Dmax 8.6 nm
VolumePorod 57 nm3

SASDG25 – DNA repair protein Rad5 (with 6xhis and twin-strep tags)

DNA repair protein RAD5 experimental SAS data
OTHER [STATIC IMAGE] model
Sample: DNA repair protein RAD5 monomer, 138 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM KCl, 5 mM DTT, 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 6
Conformational flexibility of fork-remodeling helicase Rad5 shown by full-ensemble hybrid methods. PLoS One 14(10):e0223875 (2019)
Gildenberg MS, Washington MT
RgGuinier 4.7 nm
Dmax 17.8 nm
VolumePorod 254 nm3

SASDDM9 – lipoprotein lipase–GPIHBP1 complex

Lipoprotein lipaseGlycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 experimental SAS data
DAMMIF model
Sample: Lipoprotein lipase dimer, 101 kDa Homo sapiens protein
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 dimer, 29 kDa Homo sapiens protein
Buffer: 10 mM BisTris, 150 mM NaCl, 4 mM CaCl2, 10% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 2
Structure of the lipoprotein lipase-GPIHBP1 complex that mediates plasma triglyceride hydrolysis. Proc Natl Acad Sci U S A (2018)
Birrane G, Beigneux AP, Dwyer B, Strack-Logue B, Kristensen KK, Francone OL, Fong LG, Mertens HDT, Pan CQ, Ploug M, Young SG, Meiyappan M
RgGuinier 5.5 nm
Dmax 19.0 nm
VolumePorod 319 nm3

SASDCR2 – Bromodomain-containing protein 4 (BRD4) tandem bromodomains

Bromodomain-containing protein 4 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 4 monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 251 nm3

SASDCS2 – Bromodomain-containing protein 3 (BRD3) tandem bromodomains

Bromodomain-containing protein 3 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 3 monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.2 nm
Dmax 21.5 nm
VolumePorod 210 nm3

SASDCT2 – Bromodomain-containing protein 2 (BRD2) tandem bromodomains

Bromodomain-containing protein 2 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 5.7 nm
Dmax 21.0 nm
VolumePorod 220 nm3

SASDCU2 – Bromodomain testis-specific protein (BRDT) tandem bromodomains

Bromodomain testis-specific protein experimental SAS data
DAMMIN model
Sample: Bromodomain testis-specific protein monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 5.1 nm
Dmax 20.0 nm
VolumePorod 200 nm3

SASDET8 – GTPase Elongation Factor like-1 bound Shwachman-Diamond Syndrome protein (EFL1*SDo1)

Ribosome assembly protein 1Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae protein
Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
RgGuinier 5.0 nm
Dmax 16.0 nm
VolumePorod 333 nm3

SASDED6 – Proliferating cell nuclear antigen - UbPCNA - Split Fusion Trimer

Proliferating cell nuclear antigen experimental SAS data
UIOWA_BD model
Sample: Proliferating cell nuclear antigen trimer, 117 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods. J Mol Biol 430(24):5294-5303 (2018)
Powers KT, Lavering ED, Washington MT
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 238 nm3

SASDEE6 – Proliferating cell nuclear antigen - SUMOPCNA - Split Fusion Trimer

Proliferating cell nuclear antigen experimental SAS data
UIOWA_BD model
Sample: Proliferating cell nuclear antigen trimer, 125 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods. J Mol Biol 430(24):5294-5303 (2018)
Powers KT, Lavering ED, Washington MT
RgGuinier 4.7 nm
Dmax 16.9 nm
VolumePorod 310 nm3