Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDC58 – Dipeptidyl peptidase III: pgDPP3_FL

Peptidase, M49 family experimental SAS data

Sample:	Peptidase, M49 family monomer, 102 kDa Porphyromonas gingivalis protein
Buffer:	50mM Tris,100mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 Mar 14

A novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain. PLoS One 12(11):e0188915 (2017)
Hromić-Jahjefendić A, Jajčanin Jozić N, Kazazić S, Grabar Branilović M, Karačić Z, Schrittwieser JH, Das KMP, Tomin M, Oberer M, Gruber K, Abramić M, Tomić S

R_g^Guinier	3.0	nm
D_max	11.3	nm
Volume^Porod	116	nm³

SASDAT7 – Aptamer AIR-3 5FU

Sample:	AIR3-5FU dimer, 69 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U

R_g^Guinier	4.3	nm
D_max	16.0	nm
Volume^Porod	77	nm³

SASDAU7 – Aptamer AIR-3 FdU

Sample:	AIR3-FdU dimer, 69 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	5.0	nm
D_max	16.0	nm
Volume^Porod	67	nm³

SASDBA4 – Plakin domain fragment of Human plectin (central region spectrin repeats: SR3-SR4-SR5-SR6 and SH3)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 experimental SAS data

Sample:	Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 monomer, 53 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26

The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM

R_g^Guinier	5.1	nm
D_max	21.0	nm
Volume^Porod	91	nm³

SASDBB4 – Plakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 experimental SAS data

Sample:	Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 monomer, 43 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26

R_g^Guinier	4.2	nm
D_max	17.0	nm
Volume^Porod	57	nm³

SASDBC4 – Plakin domain of Human plectin (spectrin repeats: SR3-SR9)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 experimental SAS data

Sample:	Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 monomer, 96 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 13

R_g^Guinier	8.5	nm
D_max	35.0	nm
Volume^Porod	135	nm³

SASDBD4 – Plakin domain fragment of Human desmoplakin (C-terminal region spectrin repeats: SR7-SR8-SR9)

Plakin domain fragment of Human Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 experimental SAS data

Sample:	Plakin domain fragment of Human Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 monomer, 42 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25

R_g^Guinier	4.4	nm
D_max	17.5	nm
Volume^Porod	69	nm³

SASDAP7 – PBX1-PREB1 complex

Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1 experimental SAS data

Sample:	Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding. FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C

R_g^Guinier	5.8	nm
D_max	19.0	nm
Volume^Porod	143	nm³

SASDAQ7 – PBX1-PREB1-DNA complex

Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1DNA 22mer experimental SAS data

Sample:	Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein DNA 22mer monomer, 12 kDa DNA
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	4.8	nm
D_max	16.3	nm
Volume^Porod	173	nm³

SASDAR7 – DNA 22mer

Sample:	DNA 22mer monomer, 12 kDa DNA
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	19	nm³

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