Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDU39 – Nearly full-length monomeric Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) (7-1110)

Ubiquitin carboxyl-terminal hydrolase 8 experimental SAS data

Sample:	Ubiquitin carboxyl-terminal hydrolase 8 monomer, 127 kDa Homo sapiens protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 18

Autoinhibition of ubiquitin-specific protease 8: insights into domain interactions and mechanisms of regulation Journal of Biological Chemistry :107727 (2024)
Caba C, Black M, Liu Y, DaDalt A, Mallare J, Fan L, Harding R, Wang Y, Vacratsis P, Huang R, Zhuang Z, Tong Y

R_g^Guinier	8.4	nm
D_max	31.8	nm
Volume^Porod	327	nm³

SASDV54 – YdaS, a cro-type repressor from Escherichai coli O157:H7 cryptic prophage CP-933 bound to a 22 base pair dsDNA.

Phage antirepressor protein CroIR1 22 base pair dsDNA experimental SAS data

Sample:	Phage antirepressor protein Cro dimer, 24 kDa Escherichia coli O157:H7 protein IR1 22 base pair dsDNA monomer, 14 kDa DNA
Buffer:	20 mM sodium acetate, 150 mM NaCl, 1 mM TCEP, pH: 5.5
Experiment:	SAXS data collected at BM29, ESRF on 2021 Apr 13

Structural-function relationship of YdaS, a Cro-type repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7
Marusa Prolic Kalinsek

R_g^Guinier	2.8	nm
D_max	10.1	nm
Volume^Porod	53	nm³

SASDUA2 – The Z-DNA binding domains Zαβ of Z-DNA binding protein 1 (monomer)

Z-DNA-binding protein 1 experimental SAS data

Sample:	Z-DNA-binding protein 1 monomer, 18 kDa Homo sapiens protein
Buffer:	20 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23

ZBP1 condensate formation synergizes Z-NAs recognition and signal transduction. Cell Death Dis 15(7):487 (2024)
Xie F, Wu D, Huang J, Liu X, Shen Y, Huang J, Su Z, Li J

R_g^Guinier	2.1	nm
D_max	9.0	nm
Volume^Porod	19	nm³

SASDUB2 – The Z-DNA binding domains Zαβ of Z-DNA binding protein 1 (dimer)

Sample:	Z-DNA-binding protein 1 dimer, 37 kDa Homo sapiens protein
Buffer:	20 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23

ZBP1 condensate formation synergizes Z-NAs recognition and signal transduction. Cell Death Dis 15(7):487 (2024)
Xie F, Wu D, Huang J, Liu X, Shen Y, Huang J, Su Z, Li J

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	48	nm³

SASDSP7 – 5’-terminal stem-loop 1 RNA element of SARS-CoV-2 (5_SL1)

Sample:	5_SL1 monomer, 9 kDa Severe acute respiratory … RNA
Buffer:	50 mM BisTris, 25 mM NaCl, pH: 6.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1

The 5'-terminal stem-loop RNA element of SARS-CoV-2 features highly dynamic structural elements that are sensitive to differences in cellular pH. Nucleic Acids Res (2024)
Toews S, Wacker A, Faison EM, Duchardt-Ferner E, Richter C, Mathieu D, Bottaro S, Zhang Q, Schwalbe H

R_g^Guinier	1.7	nm
D_max	5.5	nm
Volume^Porod	16	nm³