Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ensemble

SASDL36 – CBD12 of CALX1.1 in the calcium-bound state

CBD12 of CALX1.1 in the calcium-bound state Rg histogram

Sample:	AT07459p monomer, 30 kDa Drosophila melanogaster protein
Buffer:	20 mM Tris, 5 mM β-mercaptoethanol, 200 mM NaCl, 1% v/v glycerol, 0.8 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Apr 13

Molecular insights on CALX-CBD12 inter-domain dynamics from MD simulations, RDCs and SAXS. Biophys J (2021)
de Souza Degenhardt MF, Vitale PAM, Abiko LA, Zacharias M, Sattler M, Oliveira CLP, Salinas RK

R_g^Guinier	2.7	nm
D_max	9.3	nm
Volume^Porod	40	nm³

SASDLT3 – Ataxin-1 AXH-C

Sample:	Ataxin-1 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 13

A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1. J Mol Biol :167174 (2021)
Leysen S, Jane Burnley R, Rodriguez E, Milroy LG, Soini L, Adamski CJ, Nitschke L, Davis R, Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J

R_g^Guinier	4.3	nm
D_max	14.6	nm
Volume^Porod	90	nm³

SASDL69 – Ectodomain of human syndecan-1

Ectodomain of human syndecan-1 Rg histogram

Sample:	Syndecan-1 monomer, 27 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Sep 26

Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S

R_g^Guinier

5.3

SASDL79 – Ectodomain of human syndecan-2

Ectodomain of human syndecan-2 Rg histogram

Sample:	Syndecan-2 monomer, 17 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jan 31

R_g^Guinier

4.3

SASDL89 – Ectodomain of human syndecan-3

Ectodomain of human syndecan-3 Rg histogram

Sample:	Syndecan-3 monomer, 39 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 May 29

R_g^Guinier

6.5

SASDL99 – Ectodomain of human syndecan-4 isoform-2 Monomer

Ectodomain of human syndecan-4 isoform-2 Monomer Rg histogram

Sample:	Syndecan-4 monomer, 18 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Jul 4

R_g^Guinier

4.2

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 18

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K

R_g^Guinier	3.9	nm
D_max	17.3	nm
Volume^Porod	66	nm³

SASDJJ6 – Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)

N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1) Rg histogram

Sample:	N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 20 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 29

R_g^Guinier	3.5	nm
D_max	14.1	nm
Volume^Porod	46	nm³

SASDJM6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd)

Half dimer of α-actinin-2 experimental SAS data

Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd) Rg histogram

Sample:	Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.3	nm
D_max	22.0	nm
Volume^Porod	172	nm³

SASDJN6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1)

Rod domain of α-actinin-2Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	7.6	nm
D_max	28.4	nm
Volume^Porod	371	nm³

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