|
|
|
|
|
| Sample: |
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
|
| Buffer: |
50 mM Tris-HCl 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 May 18
|
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
|
| RgGuinier |
5.8 |
nm |
| Dmax |
22.5 |
nm |
| VolumePorod |
242 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
|
| Buffer: |
20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2020 Sep 25
|
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.
PLoS Pathog 17(8):e1009824 (2021)
Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC
|
| RgGuinier |
4.2 |
nm |
| Dmax |
18.1 |
nm |
| VolumePorod |
90 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
|
| Buffer: |
25 mM Tris, 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2020 Mar 3
|
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis.
Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
|
| RgGuinier |
2.8 |
nm |
| Dmax |
9.7 |
nm |
| VolumePorod |
72 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
|
| Buffer: |
25 mM Tris, 150 mM NaCl, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2020 Mar 3
|
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis.
Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
|
| RgGuinier |
2.9 |
nm |
| Dmax |
9.9 |
nm |
| VolumePorod |
83 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
|
| Buffer: |
50 mM MOPS, 50 mM NaCl, pH: 7 |
| Experiment: |
SAXS
data collected at BM29, ESRF on 2018 Dec 5
|
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA.
Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
|
| RgGuinier |
3.5 |
nm |
| Dmax |
11.8 |
nm |
| VolumePorod |
41 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
|
| Buffer: |
50 mM MOPS, 50 mM NaCl, pH: 7 |
| Experiment: |
SAXS
data collected at BM29, ESRF on 2018 Dec 5
|
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA.
Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
|
| RgGuinier |
3.8 |
nm |
| Dmax |
16.5 |
nm |
| VolumePorod |
53 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Tetanus toxin (C467S) monomer, 46 kDa Clostridium tetani protein
|
| Buffer: |
10 mM HEPES 100 mM NaCl, pH: 7.4 |
| Experiment: |
SAXS
data collected at BL45XU, SPring-8 on 2018 Oct 20
|
Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses.
J Struct Biol X 5:100045 (2021)
Zhang CM, Imoto Y, Hikima T, Inoue T
|
| RgGuinier |
3.0 |
nm |
| Dmax |
12.1 |
nm |
| VolumePorod |
54 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Testican-2 monomer, 40 kDa Homo sapiens protein
|
| Buffer: |
20 mM HEPES (Na), 130 mM NaCl, 3 mM CaCl2, 5 % (v/v) glycerol, pH: 7.4 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2016 Oct 18
|
The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration
International Journal of Molecular Sciences 21(24):9413 (2020)
Krajnc A, Gaber A, Lenarčič B, Pavšič M
|
| RgGuinier |
3.2 |
nm |
| Dmax |
12.6 |
nm |
| VolumePorod |
78 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Intimin (D0-D1 domain, 6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
|
| Buffer: |
10 mM HEPES, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 Jul 12
|
The extracellular juncture domains in the intimin passenger adopt a constitutively extended conformation inducing restraints to its sphere of action.
Sci Rep 10(1):21249 (2020)
Weikum J, Kulakova A, Tesei G, Yoshimoto S, Jægerum LV, Schütz M, Hori K, Skepö M, Harris P, Leo JC, Morth JP
|
| RgGuinier |
2.2 |
nm |
| Dmax |
5.8 |
nm |
| VolumePorod |
34 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Nucleolar RNA helicase 2 fragment 186-710 dimer, 121 kDa Homo sapiens protein
|
| Buffer: |
20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 |
| Experiment: |
SAXS
data collected at BM29, ESRF on 2018 Mar 4
|
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity
iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
|
| RgGuinier |
4.6 |
nm |
| Dmax |
17.7 |
nm |
| VolumePorod |
238 |
nm3 |
|
|
![Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram](/media/fitting_files/extra/plots/SASDJ76_fit1_extra1_rghistogram_img.png "Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram")
