Browse by MODEL: Ensemble

SASDS47 – 1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR)

1-deoxy-D-xylulose-5-phosphate reductoisomerase (G328C, K361E, S551G) experimental SAS data
1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) Rg histogram
Sample: 1-deoxy-D-xylulose-5-phosphate reductoisomerase (G328C, K361E, S551G) dimer, 104 kDa Toxoplasma gondii (strain … protein
Buffer: 20 mM Tris/HCl, 150 mM NaCl, 40 mM MgCl2, 2% glycerol,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Apr 25
1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) as target for anti Toxoplasma gondii agents: crystal structure, biochemical characterisation and biological evaluation of inhibitors Biochemical Journal (2024)
Mazzone F, Hoeppner A, Reiners J, Gertzen C, Applegate V, Abdullaziz M, Gottstein J, Degrandi D, Wesemann M, Kurz T, Smits S, Pfeffer K
RgGuinier 3.3 nm
Dmax 10.4 nm
VolumePorod 176 nm3

SASDTS4 – Type 2 DNA topoisomerase 6 subunit B-like

Type 2 DNA topoisomerase 6 subunit B-like experimental SAS data
Type 2 DNA topoisomerase 6 subunit B-like Rg histogram
Sample: Type 2 DNA topoisomerase 6 subunit B-like monomer, 61 kDa Mus musculus protein
Buffer: 20 mM HEPES, 500 mM KCl, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 12
The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization Nucleic Acids Research (2024)
Diagouraga B, Tambones I, Carivenc C, Bechara C, Nadal M, de Massy B, le Maire A, Robert T
RgGuinier 3.7 nm
Dmax 15.6 nm
VolumePorod 114 nm3

SASDUH3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

Endoglucanase-7 experimental SAS data
Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer Rg histogram
Sample: Endoglucanase-7 dimer, 60 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 4.8 nm
Dmax 21.7 nm
VolumePorod 83 nm3

SASDQ98 – Full-length human DPCD

Protein DPCD experimental SAS data
Full-length human DPCD Rg histogram
Sample: Protein DPCD monomer, 23 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2018 Nov 8
DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling. Cell Rep 43(2):113713 (2024)
Mao YQ, Seraphim TV, Wan Y, Wu R, Coyaud E, Bin Munim M, Mollica A, Laurent E, Babu M, Mennella V, Raught B, Houry WA
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 28 nm3

SASDRB8 – Human tRNA guanine transglycosylase heterodimer

Queuine tRNA-ribosyltransferase catalytic subunit 1Queuine tRNA-ribosyltransferase accessory subunit 2 experimental SAS data
Human tRNA guanine transglycosylase heterodimer Rg histogram
Sample: Queuine tRNA-ribosyltransferase catalytic subunit 1 monomer, 44 kDa Homo sapiens protein
Queuine tRNA-ribosyltransferase accessory subunit 2 monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1
Structural and functional investigation of tRNA guanine transglycosylase University of Göttingen Dissertation - (2023)
Katharina Sievers
RgGuinier 3.3 nm
Dmax 11.4 nm
VolumePorod 127 nm3

SASDRC8 – Human tRNA guanine transglycosylase bound to aspartate transfer RNA (tRNA-Asp)

Queuine tRNA-ribosyltransferase catalytic subunit 1Queuine tRNA-ribosyltransferase accessory subunit 2transfer RNA (Aspartate) experimental SAS data
Human tRNA guanine transglycosylase bound to aspartate transfer RNA (tRNA-Asp) Rg histogram
Sample: Queuine tRNA-ribosyltransferase catalytic subunit 1 monomer, 44 kDa Homo sapiens protein
Queuine tRNA-ribosyltransferase accessory subunit 2 monomer, 47 kDa Homo sapiens protein
Transfer RNA (Aspartate) monomer, 25 kDa Homo sapiens RNA
Buffer: 20 mM HEPES, 100 mM NaCl, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1
Structural and functional investigation of tRNA guanine transglycosylase University of Göttingen Dissertation - (2023)
Katharina Sievers
RgGuinier 3.5 nm
Dmax 10.9 nm
VolumePorod 160 nm3

SASDQ97 – Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E]

DNA repair protein RAD51 homolog 1 (F86E A89E) experimental SAS data
Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] Rg histogram
Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 7
Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer Angewandte Chemie International Edition (2023)
Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
RgGuinier 3.0 nm
Dmax 14.2 nm
VolumePorod 61 nm3

SASDQU9 – His-Tagged Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with His-tagged fourth BRC repeat (BRC4)

DNA repair protein RAD51 homolog 1 (F86E, A89E, His-Tagged)Breast cancer type 2 susceptibility protein experimental SAS data
His-Tagged Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with His-tagged fourth BRC repeat (BRC4) Rg histogram
Sample: DNA repair protein RAD51 homolog 1 (F86E, A89E, His-Tagged) monomer, 40 kDa Homo sapiens protein
Breast cancer type 2 susceptibility protein monomer, 6 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 7
Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer Angewandte Chemie International Edition (2023)
Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
RgGuinier 3.0 nm
Dmax 16.5 nm
VolumePorod 78 nm3

SASDQT9 – Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with fourth BRC repeat (BRC4)

DNA repair protein RAD51 homolog 1 (F86E A89E)Breast cancer type 2 susceptibility protein experimental SAS data
Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with fourth BRC repeat (BRC4) Rg histogram
Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein
Breast cancer type 2 susceptibility protein monomer, 4 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 50 mM Na2SO4, 2% v/v sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Dec 7
Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer Angewandte Chemie International Edition (2023)
Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
RgGuinier 2.9 nm
Dmax 15.6 nm
VolumePorod 70 nm3

SASDR22 – Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 1

Beclin-1 experimental SAS data
Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 1 Rg histogram
Sample: Beclin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM Tris, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 12
Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region. Autophagy :1-17 (2023)
Mukhopadhyay S, Subedi S, Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 49 nm3