Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDED7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4, 2mM Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data

Sample:	Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer:	10 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2018 Oct 20

Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA

R_g^Guinier	3.4	nm
D_max	8.9	nm
Volume^Porod	134	nm³

SASDEE7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5, 0.5 mM Calcium

Sample:	Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer:	10 mM Hepes, pH 6.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Oct 20

R_g^Guinier	3.4	nm
D_max	8.9	nm
Volume^Porod	132	nm³

SASDEF7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5 without Calcium

Sample:	Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer:	10 mM HEPES pH 6.5 150 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Oct 18

R_g^Guinier	3.4	nm
D_max	8.9	nm
Volume^Porod	140	nm³

SASDEG7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5 without Calcium

Sample:	Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein
Buffer:	10 mM Hepes, pH 4.5, 150 mM NaCl, pH: 4.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Oct 18

R_g^Guinier	3.7	nm
D_max	10.0	nm
Volume^Porod	285	nm³

SASDEH7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5, 0.5 mM Calcium

Sample:	Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein
Buffer:	10 mM Hepes, pH 4.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 4.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Oct 20

R_g^Guinier	3.7	nm
D_max	10.0	nm
Volume^Porod	280	nm³

SASDEF5 – Phosphoketolase (L. lactis) with 1 mM thiaminpyrophosphate and 2% v/v glycerol at pH 7.0

Probable phosphoketolase experimental SAS data

Sample:	Probable phosphoketolase dimer, 191 kDa Lactococcus lactis subsp. … protein
Buffer:	20mM potassium phosphate 150mM NaCl 0.007 %(w/v) β-octyl glucoside 1mM DTT 1mM MgCl 1mM thiaminpyrophosphate 2 %(v/v) glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 7

Crystal structure of a xylulose 5-phosphate phosphoketolase. insights into the substrate specificity for xylulose 5-phosphate. J Struct Biol (2019)
Scheidig AJ, Horvath D, Szedlacsek SE

R_g^Guinier	3.4	nm
D_max	10.2	nm
Volume^Porod	237	nm³

SASDEB8 – Bacillus thuringiensis LexA repressor (Bt_LexA)

Bacillus thuringiensis LexA repressor experimental SAS data

Sample:	Bacillus thuringiensis LexA repressor dimer, 47 kDa Bacillus thuringiensis protein
Buffer:	20 mM Hepes, 300 mM NaCl, 10% glycerol,, pH: 8
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Aug 25

Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor. Structure 27(7):1094-1102.e4 (2019)
Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ

R_g^Guinier	3.7	nm
Volume^Porod	110	nm³

SASDEC8 – Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)

Bacillus thuringiensis LexA repressorBacteriophage pGIL01 gp7 experimental SAS data

Sample:	Bacillus thuringiensis LexA repressor dimer, 47 kDa Bacillus thuringiensis protein Bacteriophage pGIL01 gp7 tetramer, 24 kDa Bacteriophage pGIL01 protein
Buffer:	20 mM Hepes, 300 mM NaCl, 10% glycerol,, pH: 8
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Aug 25

R_g^Guinier

4.4

SASDC45 – Alpha domain of autotransporter protein UpaB from UPEC

Alpha domain of autotransporter protein UpaB experimental SAS data

Sample:	Alpha domain of autotransporter protein UpaB monomer, 48 kDa E. Coli CFT073 protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 1

Unique structural features of a bacterial autotransporter adhesin suggest mechanisms for interaction with host macromolecules. Nat Commun 10(1):1967 (2019)
Paxman JJ, Lo AW, Sullivan MJ, Panjikar S, Kuiper M, Whitten AE, Wang G, Luan CH, Moriel DG, Tan L, Peters KM, Phan MD, Gee CL, Ulett GC, Schembri MA, Heras B

R_g^Guinier	2.9	nm
D_max	10.5	nm
Volume^Porod	66	nm³

SASDDH9 – Chloroplastic phosphoribulokinase (collected using SEC-SAXS)

Phosphoribulokinase, chloroplastic experimental SAS data

Sample:	Phosphoribulokinase, chloroplastic dimer, 78 kDa Chlamydomonas reinhardtii protein
Buffer:	Tris-HCl 50 mM 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Feb 15

Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle. Proc Natl Acad Sci U S A 116(16):8048-8053 (2019)
Gurrieri L, Del Giudice A, Demitri N, Falini G, Pavel NV, Zaffagnini M, Polentarutti M, Crozet P, Marchand CH, Henri J, Trost P, Lemaire SD, Sparla F, Fermani S

R_g^Guinier	3.4	nm
D_max	11.3	nm
Volume^Porod	115	nm³

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