Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDDM2 – Sinorhizobium meliloti Proline Utilization A (PutA) at 2.00 mg/ml

Sinorhizobium meliloti (SmPutA) experimental SAS data

Sample:	Sinorhizobium meliloti (SmPutA) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer:	50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP, and 50 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Mar 27

Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem 291(46):24065-24075 (2016)
Luo M, Gamage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ

R_g^Guinier	3.8	nm
D_max	11.9	nm
Volume^Porod	225	nm³

SASDDN2 – Sinorhizobium meliloti Proline Utilization A (PutA) at 3.00 mg/ml

Sample:	Sinorhizobium meliloti (SmPutA) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer:	50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP, and 50 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Mar 27

R_g^Guinier	3.8	nm
D_max	11.8	nm
Volume^Porod	248	nm³

SASDDP2 – Sinorhizobium meliloti Proline Utilization A (PutA) at high concentration, 4.00 mg/ml

Sample:	Sinorhizobium meliloti (SmPutA) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer:	50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP, and 50 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Mar 27

R_g^Guinier	3.9	nm
D_max	11.9	nm
Volume^Porod	277	nm³

SASDBZ5 – Enzyme I-Ntr (residues 170-424) in complex with NPr (residues 1-85)

Phosphocarrier protein NPrPhosphoenolpyruvate-protein phosphotransferase PtsP experimental SAS data

Sample:	Phosphocarrier protein NPr monomer, 9 kDa Escherichia coli protein Phosphoenolpyruvate-protein phosphotransferase PtsP monomer, 28 kDa Escherichia coli protein
Buffer:	10 mM Tris 150 mM NaCl 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2015 Jan 30

Structure of the NPr:EIN^Ntr Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems. Structure 24(12):2127-2137 (2016)
Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N

R_g^Guinier	2.3	nm
D_max	8.2	nm
Volume^Porod	52	nm³

SASDA89 – Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor

GM-CSF/IL-2 inhibition factorGranulocyte-macrophage colony-stimulating factor experimental SAS data

Sample:	GM-CSF/IL-2 inhibition factor tetramer, 120 kDa Orf virus protein Granulocyte-macrophage colony-stimulating factor dimer, 29 kDa Ovis aries protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Sep 10

Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF. Nat Commun 7:13228 (2016)
Felix J, Kandiah E, De Munck S, Bloch Y, van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN

R_g^Guinier	3.8	nm
D_max	12.4	nm
Volume^Porod	231	nm³

SASDA99 – Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor

GM-CSF/IL-2 inhibition factorInterleukin-2 experimental SAS data

Sample:	GM-CSF/IL-2 inhibition factor tetramer, 120 kDa Orf virus protein Interleukin-2 monomer, 16 kDa Ovis aries protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Sep 10

R_g^Guinier	4.1	nm
D_max	12.9	nm
Volume^Porod	253	nm³

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data

Sample:	Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI

R_g^Guinier	2.8	nm
D_max	8.2	nm
Volume^Porod	100	nm³

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data

Sample:	Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

R_g^Guinier	3.9	nm
D_max	12.7	nm
Volume^Porod	202	nm³

SASDAV7 – Aptamer AIR-3A

Sample:	AIR-3A dimer, 13 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U

R_g^Guinier	1.9	nm
D_max	6.5	nm
Volume^Porod	14	nm³

SASDAY7 – IL-6R tetramer-dimer equilibrium

Interleukin-6 receptor subunit alphaInterleukin-6 receptor subunit alpha experimental SAS data

Sample:	Interleukin-6 receptor subunit alpha tetramer, 164 kDa Homo sapiens protein Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2014 Oct 1

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	240	nm³

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