Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDT75 – Trypanosoma brucei gambiense invariant surface glycoprotein 43 (ISG43)

T. brucei spp.-specific protein experimental SAS data

Sample:	T. brucei spp.-specific protein monomer, 38 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S

R_g^Guinier	3.2	nm
D_max	11.5	nm
Volume^Porod	53	nm³

SASDT85 – Trypanosoma brucei gambiense invariant surface glycoprotein 64 (ISG64)

64 kDa invariant surface glycoprotein, putative experimental SAS data

Sample:	64 kDa invariant surface glycoprotein, putative monomer, 40 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	3.1	nm
D_max	11.2	nm
Volume^Porod	56	nm³

SASDT95 – Trypanosoma brucei gambiense invariant surface glycoprotein 75 (ISG75)

Sample:	ISG75 monomer, 50 kDa Trypanosoma brucei gambiense protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Nov 25

R_g^Guinier	3.2	nm
D_max	11.1	nm
Volume^Porod	85	nm³

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5 experimental SAS data

Sample:	Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	4.4	nm
D_max	17.2	nm
Volume^Porod	170	nm³

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1 experimental SAS data

Sample:	Variant surface glycoprotein 3.1 dimer, 103 kDa Trypanosoma brucei gambiense protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	4.7	nm
D_max	17.0	nm
Volume^Porod	149	nm³

SASDSU8 – Threonine aldolase 1 tetramer at neutral pH

L-threonine aldolase experimental SAS data

Sample:	L-threonine aldolase tetramer, 165 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 7.8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Feb 14

One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse. Nat Commun 15(1):3199 (2024)
Malatesta M, Fornasier E, Di Salvo ML, Tramonti A, Zangelmi E, Peracchi A, Secchi A, Polverini E, Giachin G, Battistutta R, Contestabile R, Percudani R

R_g^Guinier	3.7	nm
D_max	10.6	nm
Volume^Porod	209	nm³

SASDQS9 – A ssr1698 Dri1 hemoprotein, H21A variant + heme

Ssr1698 protein (H21A) experimental SAS data

Sample:	Ssr1698 protein (H21A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Dec 7

A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE

R_g^Guinier	2.0	nm
D_max	6.5	nm
Volume^Porod	28	nm³

SASDTT4 – SnoaL-like domain-containing protein apoform (SLDCP concentrated monomeric SEC peak)

SnoaL-like domain-containing protein experimental SAS data

Sample:	SnoaL-like domain-containing protein monomer, 12 kDa Sphaerobacter thermophilus (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 6

Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes. Biochim Biophys Acta Bioenerg 1865(3):149043 (2024)
Likkei K, Moldenhauer M, Tavraz NN, Egorkin NA, Slonimskiy YB, Maksimov EG, Sluchanko NN, Friedrich T

R_g^Guinier	1.5	nm
D_max	5.0	nm
Volume^Porod	18	nm³

SASDKT6 – SARS-CoV-2 non-structural protein 14 (nsp14)

Replicase polyprotein 1ab (non-structural protein 14) experimental SAS data

Sample:	Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A

R_g^Guinier	2.7	nm
D_max	9.6	nm
Volume^Porod	80	nm³

SASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data

Sample:	Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	2.9	nm
D_max	12.2	nm
Volume^Porod	73	nm³

First << Prev Page 25 of 163 Next >> Last