Browse by MODEL: Hybrid

SASDPR5 – L-methionine gamma-lyase from Clostridium sporogenes

L-methionine gamma-lyase (K272S) experimental SAS data
CHIMERA model
Sample: L-methionine gamma-lyase (K272S) tetramer, 174 kDa Clostridium sporogenes protein
Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
RgGuinier 3.7 nm
Dmax 14.5 nm
VolumePorod 211 nm3

SASDPS5 – L-methionine gamma-lyase from Clostridium sporogenes fused with S3 domain of the Vaccinia virus growth factor

L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain experimental SAS data
CHIMERA model
Sample: L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain tetramer, 183 kDa Clostridium sporogenes protein
Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
RgGuinier 5.2 nm
Dmax 17.3 nm
VolumePorod 303 nm3

SASDSH5 – UDP-glycosyltransferase 202A2 from Tetranychus urticae

UDP-glycosyltransferase 202A2 experimental SAS data
MULTIFOXS model
Sample: UDP-glycosyltransferase 202A2 monomer, 53 kDa Tetranychus urticae protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 17
Structural and functional studies reveal the molecular basis of substrate promiscuity of a glycosyltransferase originating from a major agricultural pest Journal of Biological Chemistry :105421 (2023)
Arriaza R, Abiskaroon B, Patel M, Daneshian L, Kluza A, Snoeck S, Watkins M, Hopkins J, Van Leeuwen T, Grbic M, Grbic V, Borowski T, Chruszcz M
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDSW7 – poly(GU) RNA with 12 GU repeats

polyGU RNA - (GU)12 experimental SAS data
XPLOR-NIH model
Sample: PolyGU RNA - (GU)12 monomer, 8 kDa synthetic RNA RNA
Buffer: 20 mM HEPES, 150 mM KCl, pH: 7
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 10
Solution structure of poly(UG) RNA Journal of Molecular Biology :168340 (2023)
Escobar C, Petersen R, Tonelli M, Fan L, Henzler-Wildman K, Butcher S
RgGuinier 1.3 nm
Dmax 4.7 nm
VolumePorod 8 nm3

SASDTX4 – G-actin - Skeletal muscle actin from Oryctolagus cuniculus: globular actin monomers

Actin, alpha skeletal muscle experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1
Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state Biochemical and Biophysical Research Communications :149340 (2023)
Ryzhykau Y, Povarova O, Dronova E, Kuklina D, Antifeeva I, Ilyinsky N, Okhrimenko I, Semenov Y, Kuklin A, Ivanovich V, Fonin A, Uversky V, Turoverov K, Kuznetsova I
RgGuinier 2.9 nm
Dmax 8.5 nm
VolumePorod 58 nm3

SASDSH9 – SAXS data of Clostridium perfringens enterotoxin

Heat-labile enterotoxin B chain experimental SAS data
GASBOR model
Sample: Heat-labile enterotoxin B chain monomer, 36 kDa Clostridium perfringens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18
Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A
RgGuinier 2.7 nm
Dmax 10.2 nm
VolumePorod 46 nm3

SASDSJ9 – SAXS data of trypsinized Clostridium perfringens enterotoxin

Heat-labile enterotoxin B chain experimental SAS data
GASBOR model
Sample: Heat-labile enterotoxin B chain monomer, 33 kDa Clostridium perfringens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18
Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 40 nm3

SASDTU2 – NAD-dependent protein deacetylase sirtuin-7 at pH 8.0

Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 experimental SAS data
CORAL model
Sample: Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 monomer, 45 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 16
Substrates and Cyclic Peptide Inhibitors of the Oligonucleotide-Activated Sirtuin 7. Angew Chem Int Ed Engl :e202314597 (2023)
Bolding JE, Nielsen AL, Jensen I, Hansen TN, Ryberg LA, Jameson ST, Harris P, Peters GHJ, Denu JM, Rogers JM, Olsen CA
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 89 nm3

SASDSE8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.27 mg/mL

Adhesion G-protein coupled receptor G4 experimental SAS data
MODELLER model
Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21
The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces. J Biol Chem :105356 (2023)
Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N
RgGuinier 2.7 nm
Dmax 7.1 nm
VolumePorod 67 nm3

SASDSF8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.55 mg/mL

Adhesion G-protein coupled receptor G4 experimental SAS data
MODELLER model
Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21
The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces. J Biol Chem :105356 (2023)
Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 65 nm3