Browse by MODEL: Hybrid

SASDW57 – SSRD-2xDGSt - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) and Dual SpyCatcher-SpyTag Linkages with DCV-GFP-St Extension

TET12-2Sc-RFP-DCVDCV-GFP-StDCV-GFP-St experimental SAS data
MODELLER model
Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein
DCV-GFP-St monomer, 41 kDa synthetic construct protein
DCV-GFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R
RgGuinier 5.8 nm
Dmax 17.3 nm
VolumePorod 580 nm3

SASDW67 – DRSt - Chimeric protein consisting of DCV, RFP and SpyTag domain

DCV-RFP-St experimental SAS data
MODELLER model
Sample: DCV-RFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 60 nm3

SASDW77 – DGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain

DCV-GFP-St experimental SAS data
MODELLER model
Sample: DCV-GFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 54 nm3

SASDW87 – DGSt-LucSc - DCV-GFP-SpyTag and NanoLuc-SpyCatcher Conjugate via SpyCatcher-SpyTag Reaction

DCV-GFP-StNanoLuc-Sc experimental SAS data
SREFLEX model
Sample: DCV-GFP-St monomer, 41 kDa synthetic construct protein
NanoLuc-Sc monomer, 30 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 105 nm3

SASDX54 – Precursor microRNA 20a (pre-miR-20a)

Precursor microRNA 20a experimental SAS data
AMBER model
Sample: Precursor microRNA 20a monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, 5 mM BME, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 8
Structural features within precursor microRNA-20a regulate Dicer-TRBP processing Journal of Molecular Biology :169317 (2025)
Liu Y, Harkner C, Westwood M, Munsayac A, Keane S
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 32 nm3

SASDVC7 – Tetrameric L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli

L-threonine dehydratase biosynthetic IlvA experimental SAS data
CHIMERA model
Sample: L-threonine dehydratase biosynthetic IlvA tetramer, 233 kDa Escherichia coli (strain … protein
Buffer: 20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 14
Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA). Biochemistry (2025)
Yun MK, Subramanian C, Miller K, Jackson P, Radka CD, Rock CO
RgGuinier 4.5 nm
Dmax 15.6 nm
VolumePorod 284 nm3

SASDVD7 – Regulatory domain of L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli

L-threonine dehydratase biosynthetic IlvA Regulatory domain experimental SAS data
CHIMERA model
Sample: L-threonine dehydratase biosynthetic IlvA Regulatory domain dimer, 46 kDa Escherichia coli (strain … protein
Buffer: 20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 14
Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA). Biochemistry (2025)
Yun MK, Subramanian C, Miller K, Jackson P, Radka CD, Rock CO
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 50 nm3

SASDVE7 – Hexameric L-threonine dehydratase biosynthetic (IlvA) from Staphylococcus aureus

L-threonine dehydratase biosynthetic IlvA experimental SAS data
CHIMERA model
Sample: L-threonine dehydratase biosynthetic IlvA hexamer, 288 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 14
Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA). Biochemistry (2025)
Yun MK, Subramanian C, Miller K, Jackson P, Radka CD, Rock CO
RgGuinier 5.1 nm
Dmax 17.1 nm
VolumePorod 386 nm3

SASDSX5 – Nucleosome core particle (NCP) composed of the Widom 601 DNA

Histone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Histone H3.2 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 23 kDa Xenopus laevis protein
Histone H2A dimer, 28 kDa Xenopus laevis protein
Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein
Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.3 nm
Dmax 12.3 nm
VolumePorod 350 nm3

SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)

Isoform 2 of B-cell CLL/lymphoma 7 protein family member AHistone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data
EOM/RANCH model
Sample: Isoform 2 of B-cell CLL/lymphoma 7 protein family member A monomer, 25 kDa Homo sapiens protein
Histone H3.2 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 23 kDa Xenopus laevis protein
Histone H2A dimer, 28 kDa Xenopus laevis protein
Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein
Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.7 nm
Dmax 24.4 nm
VolumePorod 400 nm3