Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDWQ5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV)

Genome polyprotein experimental SAS data

Sample:	Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Buffer:	20 mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26

A new protein-dependent riboswitch activates ribosomal frameshifting (2025)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C

R_g^Guinier	1.9	nm
D_max	7.2	nm
Volume^Porod	30	nm³

SASDWR5 – Stimulatory element RNA

Stimulatory element RNA experimental SAS data

Sample:	Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer:	20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26

R_g^Guinier	1.9	nm
D_max	8.0	nm
Volume^Porod	19	nm³

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data

Sample:	Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer:	20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	46	nm³

SASDPT5 – Penicilin-Binding Protein 5 - PBP5∆1-39

Penicillin-Binding Protein 5 - PBP5∆1-39 experimental SAS data

Sample:	Penicillin-Binding Protein 5 - PBP5∆1-39 monomer, 75 kDa Clostridioides difficile protein
Buffer:	100mM Tris·HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SAXS of the Penicillin-Binding Protein 5 from Clostridioides difficile
Guillem Hernandez

R_g^Guinier	3.8	nm
D_max	15.0	nm
Volume^Porod	109	nm³

SASDQY7 – StyI glutathione S-transferase

GST N-terminal domain-containing protein experimental SAS data

Sample:	GST N-terminal domain-containing protein dimer, 60 kDa Gordonia rubripertincta protein
Buffer:	10 mM Tris, 300 mM NaCl,, pH: 8
Experiment:	SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2022 Jan 20

Glutathione S -Transferase Mediated Epoxide Conversion: Functional and Structural Properties of an Enantioselective Catalyst ACS Catalysis :12308-12324 (2025)
Haarmann M, Scholz M, Lienkamp A, Burnik J, Justesen B, Reiners J, Maier A, Eggerichs D, Vocke K, Smits S, Günther Pomorski T, Hofmann E, Tischler D

R_g^Guinier	2.7	nm
D_max	9.2	nm
Volume^Porod	91	nm³

SASDW94 – Horse apoferritin from AF4-SAXS measurements

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 480 kDa Equus caballus protein
Buffer:	phosphate buffered saline, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA

R_g^Guinier	5.4	nm
D_max	13.3	nm
Volume^Porod	632	nm³

SASDWA4 – Yeast alcohol dehydrogenase 1 from AF4-SAXS measurements

Alcohol dehydrogenase 1 experimental SAS data

Sample:	Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13

R_g^Guinier	3.4	nm
D_max	9.5	nm
Volume^Porod	197	nm³

SASDWB4 – Sweet potato beta-amylase from AF4-SAXS measurement

Sample:	Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer:	phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13

R_g^Guinier	4.2	nm
D_max	12.6	nm
Volume^Porod	297	nm³

SASDW37 – SSRD - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV)

Sample:	TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein
Buffer:	20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24

Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R

R_g^Guinier	4.4	nm
D_max	13.6	nm
Volume^Porod	300	nm³

SASDW47 – SSRD-2xDRSt - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) and Dual SpyCatcher-SpyTag Linkages with DCV-RFP-St Extension

TET12-2Sc-RFP-DCVDCV-RFP-StDCV-RFP-St experimental SAS data

Sample:	TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein DCV-RFP-St monomer, 41 kDa synthetic construct protein DCV-RFP-St monomer, 41 kDa synthetic construct protein
Buffer:	20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24

R_g^Guinier	5.1	nm
D_max	15.4	nm
Volume^Porod	480	nm³

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