Browse by MACROMOLECULE type: nucleic acid

SASDQG7 – Thermoanearobacter tengcongensis (Tte) fecB RNA riboswitch aptamer domain (rCbl)

Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain experimental SAS data
OTHER model
Sample: Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain, 68 kDa marine metagenome RNA
Buffer: 50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, pH: 6
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Apr 18
Visualizing RNA conformational and architectural heterogeneity in solution. Nat Commun 14(1):714 (2023)
Ding J, Lee YT, Bhandari Y, Schwieters CD, Fan L, Yu P, Tarosov SG, Stagno JR, Ma B, Nussinov R, Rein A, Zhang J, Wang YX
RgGuinier 5.9 nm
Dmax 20.7 nm
VolumePorod 206 nm3

SASDQH7 – Thermoanearobacter tengcongensis (Tte) fecB RNA riboswitch aptamer domain (rCbl) in the presence of coenzyme B12

Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain experimental SAS data
OTHER model
Sample: Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain, 68 kDa marine metagenome RNA
Buffer: 50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, 4-18 μM coenzyme B12 ligand, pH: 6
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Apr 18
Visualizing RNA conformational and architectural heterogeneity in solution. Nat Commun 14(1):714 (2023)
Ding J, Lee YT, Bhandari Y, Schwieters CD, Fan L, Yu P, Tarosov SG, Stagno JR, Ma B, Nussinov R, Rein A, Zhang J, Wang YX
RgGuinier 5.7 nm
Dmax 19.7 nm
VolumePorod 230 nm3

SASDPU6 – Duplex-G4-duplex DNA

Duplex-G4-duplex experimental SAS data
AMBER model
Sample: Duplex-G4-duplex monomer, 29 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 16
Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 Å resolution with cryo-EM. Nucleic Acids Res (2023)
Monsen RC, Chua EYD, Hopkins JB, Chaires JB, Trent JO
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 59 nm3

SASDQG5 – DNA (Zinc finger protein 410 recognition sequence)

DNA (Zinc finger protein 410 recognition sequence) experimental SAS data
DNA (Zinc finger protein 410 recognition sequence) Kratky plot
Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
Kaur G, Ren R, Hammel M, Horton JR, Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 16 nm3

SASDR82 – Alpha cyclodextrin - 50 mg/ml aqueous solution measured to 235 inverse nm

alpha cyclodextrin experimental SAS data
alpha cyclodextrin Kratky plot
Sample: Alpha cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.6 nm

SASDR92 – Alpha cyclodextrin - 25 mg/ml aqueous solution measured to 235 inverse nm

alpha cyclodextrin experimental SAS data
alpha cyclodextrin Kratky plot
Sample: Alpha cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.6 nm

SASDRA2 – Alpha cyclodextrin - 12.5 mg/ml aqueous solution measured to 235 inverse nm

alpha cyclodextrin experimental SAS data
alpha cyclodextrin Kratky plot
Sample: Alpha cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.6 nm

SASDRB2 – Beta cyclodextrin - 18 mg/ml aqueous solution measured to 238 inverse nm

Beta Cyclodextrin experimental SAS data
Beta Cyclodextrin Kratky plot
Sample: Beta Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDRC2 – Beta cyclodextrin - 12.5 mg/ml aqueous solution measured to 238 inverse nm

Beta Cyclodextrin experimental SAS data
Beta Cyclodextrin Kratky plot
Sample: Beta Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDRD2 – Gamma cyclodextrin - 50 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm