Browse by MACROMOLECULE type: protein

SASDJS4 – Type 3 secretion system pilotin, InvH70-147

Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods. Structure (2020)
Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 29 nm3

SASDJC6 – Human Vitamin K-dependent protein C

Vitamin K-dependent protein C experimental SAS data
DAMFILT model
Sample: Vitamin K-dependent protein C monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 145 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 14
Zymogen and activated protein C have similar structural architecture. J Biol Chem (2020)
Stojanovski BM, Pelc LA, Zuo X, Di Cera E
RgGuinier 3.6 nm
Dmax 14.0 nm

SASDJD6 – Human Vitamin K-dependent Activated Protein C

Vitamin K-dependent protein C experimental SAS data
DAMFILT model
Sample: Vitamin K-dependent protein C monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 145 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Aug 17
Zymogen and activated protein C have similar structural architecture. J Biol Chem (2020)
Stojanovski BM, Pelc LA, Zuo X, Di Cera E
RgGuinier 3.7 nm
Dmax 14.0 nm

SASDJS3 – Adenylate cyclase toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 94 nm3

SASDJT3 – Adenylate cyclase toxin CyaA RTX block III-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 3.4 nm
Dmax 12.1 nm
VolumePorod 85 nm3

SASDJU3 – Adenylate cyclase toxin CyaA RTX block IV-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 45 nm3

SASDJA4 – Endonuclease VIII from E. coli (size exclusion chromatography SAXS)

Endonuclease 8 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endonuclease 8 monomer, 31 kDa Escherichia coli protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments. Structure (2020)
Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 45 nm3

SASDJB4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) full length

Nei like DNA glycosylase 2 experimental SAS data
Nei like DNA glycosylase 2 Kratky plot
Sample: Nei like DNA glycosylase 2 monomer, 39 kDa Monodelphis domestica protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments. Structure (2020)
Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 58 nm3

SASDJC4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133)

Nei like DNA glycosylase 2 (Δ67-133) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nei like DNA glycosylase 2 (Δ67-133) monomer, 33 kDa Monodelphis domestica protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments. Structure (2020)
Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 47 nm3

SASDJW5 – Plasminogen activator inhibitor-1 (PAI-1) W175F bound to nanobody Nb93

Plasminogen activator inhibitor 1VHH-s-a93 (Ig module Nb93) experimental SAS data
OTHER model
Sample: Plasminogen activator inhibitor 1 monomer, 43 kDa Homo sapiens protein
VHH-s-a93 (Ig module Nb93) monomer, 13 kDa Vicugna pacos protein
Buffer: 30 mM BIS-TRIS pH 5.5, 300 mM sodium chloride, 5% v/v glycerol, pH: 5.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 5
Structural Insights into the Mechanism of a Nanobody That Stabilizes PAI-1 and Modulates Its Activity International Journal of Molecular Sciences 21(16):5859 (2020)
Sillen M, Weeks S, Strelkov S, Declerck P
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 80 nm3