Browse by ORGANISM: Homo sapiens (Human)

SASDBQ9 – Human Hsp90 co-chaperone Cdc37 (CD37) in complex with fibroblast growth factor receptor 3

Hsp90 co-chaperone Cdc37Fibroblast growth factor receptor 3 experimental SAS data
CORAL model
Sample: Hsp90 co-chaperone Cdc37 monomer, 44 kDa Homo sapiens protein
Fibroblast growth factor receptor 3 monomer, 35 kDa Homo sapiens protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10
Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System. Structure 26(3):446-458.e8 (2018)
Bunney TD, Inglis AJ, Sanfelice D, Farrell B, Kerr CJ, Thompson GS, Masson GR, Thiyagarajan N, Svergun DI, Williams RL, Breeze AL, Katan M
RgGuinier 4.7 nm
Dmax 19.5 nm
VolumePorod 161 nm3

SASDBR9 – Human fibroblast growth factor receptor 3, from SEC-SAXS

Fibroblast growth factor receptor 3 experimental SAS data
CORAL model
Sample: Fibroblast growth factor receptor 3 monomer, 35 kDa Homo sapiens protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10
Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System. Structure 26(3):446-458.e8 (2018)
Bunney TD, Inglis AJ, Sanfelice D, Farrell B, Kerr CJ, Thompson GS, Masson GR, Thiyagarajan N, Svergun DI, Williams RL, Breeze AL, Katan M
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 59 nm3

SASDC27 – Saposin nanoparticle (SapNP) SapA:DOPC

saposin-a1,2-dioleoyl-sn-glycero-3-phosphocholine experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1,2-dioleoyl-sn-glycero-3-phosphocholine, 47 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDC37 – Saposin nanoparticle (SapNP) SapA:SoyPI

saposin-aL-α-phosphatidylinositol (soy) experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
L-α-phosphatidylinositol (soy), 52 kDa Glycine max
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 3.6 nm
Dmax 8.5 nm
VolumePorod 68 nm3

SASDC47 – Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol), 46 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 10.5 nm
VolumePorod 69 nm3

SASDCX6 – T2 SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineMechanosensitive channel T2 experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine, 47 kDa synthetic construct
Mechanosensitive channel T2 heptamer, 230 kDa Thermoplasma volcanium protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 5.4 nm
Dmax 18.0 nm
VolumePorod 686 nm3

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineDipeptide and tripeptide permease A experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine, 47 kDa synthetic construct
Dipeptide and tripeptide permease A monomer, 51 kDa Escherichia coli protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 288 nm3

SASDCA3 – Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence

Zinc finger and BTB domain-containing protein 38methylated C-terminal ZBTB38 binding sequence experimental SAS data
MONSA model
Sample: Zinc finger and BTB domain-containing protein 38 monomer, 17 kDa Homo sapiens protein
Methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Nov 11
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
Pozner A, Hudson NO, Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 50 nm3

SASDCG7 – Lys63-linked diubiquitin at pH7.4

Polyubiquitin-C experimental SAS data
Polyubiquitin-C Kratky plot
Sample: Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Mar 24
Characterizing Protein Dynamics with Integrative Use of Bulk and Single-Molecule Techniques. Biochemistry 57(3):305-313 (2018)
Liu Z, Gong Z, Cao Y, Ding YH, Dong MQ, Lu YB, Zhang WP, Tang C
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 22 nm3

SASDD86 – Full-length human NHLRC2 ( NHL repeat-containing protein 2 )

NHL repeat-containing protein 2 experimental SAS data
DAMFILT model
Sample: NHL repeat-containing protein 2 monomer, 80 kDa Homo sapiens protein
Buffer: 20 mM BisTris, 100 mM NaCl, 2 mM DTT, pH: 6.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jul 8
Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease. PLoS One 13(8):e0202391 (2018)
Biterova E, Ignatyev A, Uusimaa J, Hinttala R, Ruddock LW
RgGuinier 3.6 nm
Dmax 14.7 nm
VolumePorod 121 nm3