Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDAK4 – CRM1 RanGTP

Exportin-1GTP-binding nuclear protein Ran experimental SAS data

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3

Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21(8):1350-60 (2013)
Dölker N, Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A

R_g^Guinier	3.6	nm
D_max	10.0	nm

SASDAL4 – CRM1 RanGTP Snu1

Exportin-1GTP-binding nuclear protein RanSnurportin-1 experimental SAS data

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein Snurportin-1 monomer, 41 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3

R_g^Guinier	4.1	nm
D_max	14.0	nm

SASDAM4 – CRM1 Snu1

Exportin-1Snurportin-1 experimental SAS data

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein Snurportin-1 monomer, 41 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 10

R_g^Guinier	4.3	nm
D_max	15.0	nm

SASDLV4 – Ceruloplasmin ternary complex with lactoferrin and myeloperoxidase

CeruloplasminLactotransferrinMyeloperoxidase experimental SAS data

Sample:	Ceruloplasmin dimer, 244 kDa Homo sapiens protein Lactotransferrin dimer, 156 kDa Homo sapiens protein Myeloperoxidase dimer, 132 kDa Homo sapiens protein
Buffer:	0.1 M sodium-acetate buffer, pH: 5.6
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Nov 24

Ceruloplasmin: Macromolecular Assemblies with Iron-Containing Acute Phase Proteins PLoS ONE 8(7):e67145 (2013)
Samygina V, Sokolov A, Bourenkov G, Petoukhov M, Pulina M, Zakharova E, Vasilyev V, Bartunik H, Svergun D, Tuma R

R_g^Guinier	6.9	nm
D_max	22.6	nm
Volume^Porod	726	nm³

SASDL47 – Oligomeric composition of AXH Domain of Ataxin-1 (wild type and A567G, I580A mutants)

Sample:	Ataxin-1 monomer, 14 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 21

Self-assembly and conformational heterogeneity of the AXH domain of ataxin-1: an unusual example of a chameleon fold. Biophys J 104(6):1304-13 (2013)
de Chiara C, Rees M, Menon RP, Pauwels K, Lawrence C, Konarev PV, Svergun DI, Martin SR, Chen YW, Pastore A

R_g^Guinier

5.5

SASDAG4 – HsMfe2

Peroxisomal multifunctional enzyme type 2 experimental SAS data

Peroxisomal multifunctional enzyme type 2 Kratky plot

Sample:	Peroxisomal multifunctional enzyme type 2 dimer, 159 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 22

Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
Mehtälä ML, Haataja TJ, Blanchet CE, Hiltunen JK, Svergun DI, Glumoff T

R_g^Guinier	4.6	nm
D_max	15.0	nm

SASDHY2 – Human APPL2 (DCC-interacting protein 13-beta)

Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 experimental SAS data

Sample:	Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 dimer, 87 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 7 Apr 20

Membrane curvature protein exhibits interdomain flexibility and binds a small GTPase. J Biol Chem 287(49):40996-1006 (2012)
King GJ, Stöckli J, Hu SH, Winnen B, Duprez WG, Meoli CC, Junutula JR, Jarrott RJ, James DE, Whitten AE, Martin JL

R_g^Guinier	5.1	nm
D_max	18.0	nm
Volume^Porod	140	nm³

SASDAT4 – uPAR wild-type

Urokinase plasminogen activator surface receptor experimental SAS data

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10

A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M

R_g^Guinier	2.8	nm
D_max	9.7	nm
Volume^Porod	69	nm³

SASDAU4 – uPAR H47C/N259C

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	57	nm³

SASDAV4 – uPAR wild-type AE105 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE105 experimental SAS data

Urokinase plasminogen activator surface receptor synthetic peptide AE105 Kratky plot

Sample:	Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Synthetic peptide AE105 monomer, 1 kDa protein
Buffer:	25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10

R_g^Guinier	2.5	nm
D_max	8.5	nm
Volume^Porod	61	nm³

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