Browse by ORGANISM: Homo sapiens (Human)

SASDVE9 – 7SL Signal Recognition Particle

7SL Signal Reocgnition Particle RNA experimental SAS data
DAMMIN model
Sample: 7SL Signal Reocgnition Particle RNA monomer, 98 kDa Homo sapiens RNA
Buffer: 10 mM Bis-Tris, 100 mM NaCl, 15 mM KCl, 5% Glycerol, 3 mM MgCl2, pH: 5.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 21
Solution Structure Determination and Biophysical Studies of 7SK RNP and 7SL SRP RNAs. RNA (2025)
D'Souza MH, Pereira HS, Tersteeg S, Vasudeva G, Siddiqui MQ, Agunlejika T, Kerr L, Girodat D, Patel T
RgGuinier 6.7 nm
Dmax 21.7 nm
VolumePorod 300 nm3

SASDWL2 – C-terminal mini-Collagen VI heterotrimer

Collagen alpha-1(VI) chainCollagen alpha-2(VI) chain (R680H, K966N, Q967E)Collagen alpha-3(VI) chain (D2357R, K2367R, D2431V, R2441T, R2609A, R2610A) experimental SAS data
ALPHAFOLD model
Sample: Collagen alpha-1(VI) chain, 52 kDa Homo sapiens protein
Collagen alpha-2(VI) chain (R680H, K966N, Q967E), 53 kDa Homo sapiens protein
Collagen alpha-3(VI) chain (D2357R, K2367R, D2431V, R2441T, R2609A, R2610A), 54 kDa Homo sapiens protein
Buffer: Tris-buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Jul 27
Collagen VI Microfibril Structure Reveals the Mechanism for Heterotrimerisation and the Clustering of Inherited Pathogenic Mutations
Mark H. Becker
RgGuinier 4.8 nm
Dmax 18.7 nm
VolumePorod 478 nm3

SASDVV2 – Fas-activated serine/threonine kinase/phosphoprotein FASTK (76-549)

Fas-activated serine/threonine kinase (amino acids 76-549) experimental SAS data
Sample: Fas-activated serine/threonine kinase (amino acids 76-549) monomer, 53 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 31
Human FASTK preferentially binds single-stranded and G-rich RNA. FEBS J (2025)
Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW
RgGuinier 3.6 nm
Dmax 16.3 nm
VolumePorod 96 nm3

SASDVW2 – Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)

Fas-activated serine/threonine kinase (amino acids 169-549) experimental SAS data
Sample: Fas-activated serine/threonine kinase (amino acids 169-549) monomer, 43 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 31
Human FASTK preferentially binds single-stranded and G-rich RNA. FEBS J (2025)
Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 67 nm3

SASDX54 – Precursor microRNA 20a (pre-miR-20a)

Precursor microRNA 20a experimental SAS data
AMBER model
Sample: Precursor microRNA 20a monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, 5 mM BME, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 8
Structural features within precursor microRNA-20a regulate Dicer-TRBP processing Journal of Molecular Biology :169317 (2025)
Liu Y, Harkner C, Westwood M, Munsayac A, Keane S
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 32 nm3

SASDV57 – Full-length SFPQ in high salt buffer

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Oct 22
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 7.9 nm
Dmax 42.5 nm
VolumePorod 485 nm3

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data
Splicing Factor Proline/Glutamine rich (1-598) Kratky plot
Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 7.6 nm
Dmax 34.3 nm
VolumePorod 400 nm3

SASDV77 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) in low salt buffer

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data
Splicing Factor Proline/Glutamine rich (1-598) Kratky plot
Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 6.1 nm
Dmax 28.0 nm
VolumePorod 315 nm3

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 6.1 nm
Dmax 22.0 nm
VolumePorod 181 nm3

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH: 7.4
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 23
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 8.7 nm
Dmax 30.7 nm
VolumePorod 241000 nm3