Browse by ORGANISM: Homo sapiens (Human)

SASDME5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and Ras-related protein M-Ras

Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunitRas-related protein M-Ras experimental SAS data
BILBOMD model
Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein
Ras-related protein M-Ras monomer, 24 kDa Homo sapiens protein
Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20
Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J
RgGuinier 3.5 nm
Dmax 12.4 nm
VolumePorod 215 nm3

SASDP93 – K48-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 2.6 nm
Dmax 95.0 nm
VolumePorod 44 nm3

SASDPA3 – K63-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 44 nm3

SASDPB3 – M1-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 46 nm3

SASDPC3 – HOTag6 tetramerization domain followed by a 10-Gly linker and monoubiquitin

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 115 nm3

SASDN55 – A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition with CaCl2

Diacylglycerol kinase alpha experimental SAS data
DAMMIF model
Sample: Diacylglycerol kinase alpha monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM TrisHCl, 200 mM NaCl, 5 mM DTT, 5% v/v glycerol, 3 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Dec 8
Ca 2+ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha Protein Science 31(7) (2022)
Takahashi D, Yonezawa K, Okizaki Y, Caaveiro J, Ueda T, Shimada A, Sakane F, Shimizu N
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 44 nm3

SASDN65 – A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition without CaCl2

Diacylglycerol kinase alpha experimental SAS data
DAMMIF model
Sample: Diacylglycerol kinase alpha monomer, 22 kDa Homo sapiens protein
Buffer: 20mM TrisHCl, 200mM NaCl, 5mM DTT, 5% v/v Glycerol, 3mM EGTA, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Dec 8
Ca 2+ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha Protein Science 31(7) (2022)
Takahashi D, Yonezawa K, Okizaki Y, Caaveiro J, Ueda T, Shimada A, Sakane F, Shimizu N
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 44 nm3

SASDNJ7 – Non-pre-microRNA stem loop 2 (NPSL2)

Non-pre-microRNA stem loop 2 experimental SAS data
PYMOL model
Sample: Non-pre-microRNA stem loop 2 monomer, 14 kDa Homo sapiens RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
Solution structure of NPSL2, a regulatory element in the oncomiR-1 RNA. J Mol Biol :167688 (2022)
Liu Y, Munsayac A, Hall I, Keane SC
RgGuinier 2.0 nm
Dmax 7.7 nm
VolumePorod 15 nm3

SASDNL2 – LincRNA-p21 Sense Alu Inverted Repeat

LincRNA-p21 AluSx1 Sense RNA experimental SAS data
DAMMIN model
Sample: LincRNA-p21 AluSx1 Sense RNA monomer, 100 kDa Homo sapiens RNA
Buffer: 50mM HEPES,150 mM NaCl, 15 mM MgCl2, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jul 1
Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats. Nucleic Acids Res 50(10):5881-5898 (2022)
D'Souza MH, Mrozowich T, Badmalia MD, Geeraert M, Frederickson A, Henrickson A, Demeler B, Wolfinger MT, Patel TR
RgGuinier 6.1 nm
Dmax 18.5 nm
VolumePorod 225 nm3

SASDNM2 – LincRNA-p21 Antisense Alu Inverted Repeat

LincRNA-p21 AluSx1 Antisense RNA experimental SAS data
DAMMIN model
Sample: LincRNA-p21 AluSx1 Antisense RNA monomer, 90 kDa Homo sapiens RNA
Buffer: 50mM HEPES,150 mM NaCl, 15 mM MgCl2, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jul 1
Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats. Nucleic Acids Res 50(10):5881-5898 (2022)
D'Souza MH, Mrozowich T, Badmalia MD, Geeraert M, Frederickson A, Henrickson A, Demeler B, Wolfinger MT, Patel TR
RgGuinier 5.9 nm
Dmax 18.1 nm
VolumePorod 375 nm3