Browse by ORGANISM: Homo sapiens (Human)

SASDME4 – Human tyrosyl tRNA synthetase

Tyrosine--tRNA ligase, cytoplasmic experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase, cytoplasmic dimer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
Xie S, Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S, Kosasih C, Newman W, Niles J, Lee M, Baum J, Ottilie S, Winzeler E, Creek D, Williamson N, Parker M, Brand S, Langston S, Dick L, Griffin M, Gould A, Tilley L
RgGuinier 3.6 nm
Dmax 11.5 nm
VolumePorod 118 nm3

SASDNC7 – miniCERTL with N-terminal His-tag

Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.7 nm
Dmax 9.1 nm
VolumePorod 71 nm3

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 3.3 nm
Dmax 13.6 nm
VolumePorod 84 nm3

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 158 nm3

SASDLJ7 – Human potassium voltage-gated (Kv2.1) channel T1 domain at pH8.0

Potassium voltage-gated channel subfamily B member 1 experimental SAS data
Potassium voltage-gated channel subfamily B member 1 Kratky plot
Sample: Potassium voltage-gated channel subfamily B member 1 pentamer, 73 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% v/v glycerol, 0.01% Triton X-100, 10 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Jul 13
Pentameric assembly of the Kv2.1 tetramerization domain Acta Crystallographica Section D Structural Biology 78(6):792-802 (2022)
Xu Z, Khan S, Schnicker N, Baker S
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 140 nm3

SASDLM8 – Human apo Nocturnin - Extended

Nocturnin experimental SAS data
Human apo Nocturnin - Extended Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.9 nm
Dmax 12.3 nm
VolumePorod 85 nm3

SASDLN8 – Human apo Nocturnin - Compact

Nocturnin experimental SAS data
Human apo Nocturnin - Compact Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 76 nm3

SASDLR8 – Human apo Nocturnin - N terminus truncated (118-NOCT)

Nocturnin - N terminus truncated (118-NOCT) experimental SAS data
EOM/RANCH model
Sample: Nocturnin - N terminus truncated (118-NOCT) monomer, 36 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.3 nm
Dmax 10.3 nm
VolumePorod 63 nm3

SASDND6 – Human ganglioside-induced differentiation-associated protein 1 (GDAP1) missense mutant H123R, construct GDAP1∆302-358

Ganglioside-induced differentiation-associated protein 1 (H123R) experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1 (H123R) dimer, 70 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Oct 8
Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio (2022)
Sutinen A, Nguyen GTT, Raasakka A, Muruganandam G, Loris R, Ylikallio E, Tyynismaa H, Bartesaghi L, Ruskamo S, Kursula P
RgGuinier 3.1 nm
Dmax 9.9 nm
VolumePorod 107 nm3

SASDNE6 – Human ganglioside-induced differentiation-associated protein 1 (GDAP1) missense mutant R120W, construct GDAP1∆302-358

Ganglioside-induced differentiation-associated protein 1 (R120W) experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1 (R120W) dimer, 70 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 1
Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio (2022)
Sutinen A, Nguyen GTT, Raasakka A, Muruganandam G, Loris R, Ylikallio E, Tyynismaa H, Bartesaghi L, Ruskamo S, Kursula P
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 105 nm3