Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDNR3 – Fibrillin-1 fragment PF3

Sample:	Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2016 Dec 12

Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biol (2022)
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C

R_g^Guinier	5.4	nm
D_max	24.0	nm
Volume^Porod	141	nm³

SASDNS3 – Latent-transforming growth factor beta-binding protein 1 C-terminus

Latent-transforming growth factor beta-binding protein 1 experimental SAS data

Sample:	Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2016 Dec 12

R_g^Guinier	4.7	nm
D_max	20.5	nm
Volume^Porod	102	nm³

SASDNT3 – Fibrillin-1 fragment PF3 crosslinked to latent transforming growth factor-beta-binding protein 1 C-terminus by transglutaminase 2

Latent-transforming growth factor beta-binding protein 1Fibrillin-1 PF3 experimental SAS data

Sample:	Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein
Buffer:	10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 20

R_g^Guinier	7.4	nm
D_max	29.6	nm
Volume^Porod	759	nm³

SASDKS4 – Teneurin-4 dimer wildtype in SEC buffer (20 mM HEPES, 150 mM NaCl)

Teneurin-4 dimer wildtype in SEC buffer (20 mM HEPES, 150 mM NaCl) Rg histogram

Sample:	Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

Teneurin4 dimer structures reveal a calcium‐stabilized compact conformation supporting homomeric trans‐interactions The EMBO Journal (2022)
Meijer D, Frias C, Beugelink J, Deurloo Y, Janssen B

R_g^Guinier	7.8	nm
D_max	32.0	nm
Volume^Porod	1280	nm³

SASDKT4 – Teneurin-4 dimer wildtype in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2)

Teneurin-4 dimer wildtype in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2) Rg histogram

Sample:	Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	7.2	nm
D_max	29.5	nm
Volume^Porod	1270	nm³

SASDKU4 – Teneurin-4 dimer wildtype in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA)

Teneurin-4 dimer wildtype in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA) Rg histogram

Sample:	Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM EDTA, pH: 7.8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	9.5	nm
D_max	40.0	nm
Volume^Porod	1370	nm³

SASDKV4 – Teneurin-4 dimer mutant in SEC buffer (20 mM HEPES, 150 mM NaCl)

Teneurin-4 (S2585C) experimental SAS data

Teneurin-4 dimer mutant in SEC buffer (20 mM HEPES, 150 mM NaCl) Rg histogram

Sample:	Teneurin-4 (S2585C) dimer, 537 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	7.1	nm
D_max	29.5	nm
Volume^Porod	1260	nm³

SASDKW4 – Teneurin-4 dimer mutant in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2)

Teneurin-4 dimer mutant in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2) Rg histogram

Sample:	Teneurin-4 (S2585C) dimer, 537 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	7.0	nm
D_max	29.5	nm
Volume^Porod	1270	nm³

SASDKX4 – Teneurin-4 dimer mutant in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA)

Teneurin-4 dimer mutant in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA) Rg histogram

Sample:	Teneurin-4 (S2585C) dimer, 537 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM EDTA, pH: 7.8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	7.8	nm
D_max	31.0	nm
Volume^Porod	1390	nm³

SASDMY7 – Poly-histidine tagged Myosin X component at 8979 eV

Unconventional myosin-X component experimental SAS data

Unconventional myosin-X component Kratky plot

Sample:	Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer:	HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16

K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
Virk K, Yonezawa K, Choukate K, Singh L, Shimizu N, Chaudhuri B

R_g^Guinier	2.2	nm
D_max	8.5	nm
Volume^Porod	24	nm³

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