Browse by ORGANISM: Homo sapiens (Human)

SASDHV4 – Mitochondrial complex I assembly factor, NDUFAF1

Complex I intermediate-associated protein 30, mitochondrial experimental SAS data
DAMMIN model
Sample: Complex I intermediate-associated protein 30, mitochondrial monomer, 35 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 29
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 3.3 nm
Dmax 9.6 nm
VolumePorod 82 nm3

SASDHW4 – Mitochondrial Complex I assembly factor, ACAD9

Complex I assembly factor ACAD9, mitochondrial experimental SAS data
DAMMIN model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 132 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 28
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 3.6 nm
Dmax 10.2 nm
VolumePorod 217 nm3

SASDHX4 – Mitochondrial complex I assembly factor, ACAD9, chimeric construct where segment 445-482 has been replaced with segment 481-518 of VLCAD protein

Complex I assembly factor ACAD9-VLCAD chimera experimental SAS data
DAMFILT model
Sample: Complex I assembly factor ACAD9-VLCAD chimera dimer, 131 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 30
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 3.9 nm
Dmax 11.7 nm
VolumePorod 217 nm3

SASDHY4 – Mitochondrial very long-chain specific acyl-CoA dehydrogenase enzyme, VLCAD

Very long-chain specific acyl-CoA dehydrogenase, mitochondrial experimental SAS data
DAMFILT model
Sample: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial dimer, 126 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 2
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 3.4 nm
Dmax 9.9 nm
VolumePorod 215 nm3

SASDHZ4 – The C-terminal domain of ESCIT bound to a dimer of the complex I assembly factor ACAD9

Complex I assembly factor ACAD9, mitochondrialEvolutionarily conserved signaling intermediate in Toll pathway, mitochondrial experimental SAS data
GASBOR model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 132 kDa Homo sapiens protein
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial tetramer, 88 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 4
Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination. Angew Chem Int Ed Engl (2020)
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
RgGuinier 6.0 nm
Dmax 16.5 nm
VolumePorod 683 nm3

SASDKJ2 – Testican-2 (23-379) Ca-free

Testican-2 experimental SAS data
DAMMIN model
Sample: Testican-2 monomer, 40 kDa Homo sapiens protein
Buffer: 20 mM HEPES (Na), 130 mM NaCl, 5 % (v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 18
The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration International Journal of Molecular Sciences 21(24):9413 (2020)
Krajnc A, Gaber A, Lenarčič B, Pavšič M
RgGuinier 3.4 nm
Dmax 14.0 nm
VolumePorod 82 nm3

SASDKK2 – Testican-2 (23-379) Ca-loaded

Testican-2 experimental SAS data
Testican-2 (23-379) Ca-loaded Rg histogram
Sample: Testican-2 monomer, 40 kDa Homo sapiens protein
Buffer: 20 mM HEPES (Na), 130 mM NaCl, 3 mM CaCl2, 5 % (v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 18
The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration International Journal of Molecular Sciences 21(24):9413 (2020)
Krajnc A, Gaber A, Lenarčič B, Pavšič M
RgGuinier 3.2 nm
Dmax 12.6 nm
VolumePorod 78 nm3

SASDJY3 – Immunoglobulin HUI-018 Fab in complex with human insulin

Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 86 nm3

SASDJZ3 – Immunoglobulin HUI-018 Fab in complex with human insulin at high concentration

Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.7 nm
Dmax 13.4 nm
VolumePorod 105 nm3

SASDGU9 – Nucleolar RNA helicase 2 (DDX21-FL)

Nucleolar RNA helicase 2 experimental SAS data
DAMMIF model
Sample: Nucleolar RNA helicase 2 dimer, 182 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 25
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
RgGuinier 7.0 nm
Dmax 31.9 nm
VolumePorod 681 nm3