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SASDFB9 – Poly-adenosine rA30 in 100 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.4 nm
Dmax 10.7 nm
VolumePorod 14 nm3

SASDFC9 – Poly-adenosine rA30 in 200 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.5 nm
VolumePorod 14 nm3

SASDFD9 – Poly-adenosine rA30 in 400 mM NaCl

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 13 nm3

SASDFE9 – Poly-adenosine rA30 in 600 mM NaCl

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm
VolumePorod 13 nm3

SASDFF9 – Poly-adenosine rA30 in 1 mM MgCl2

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Jul 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.4 nm
VolumePorod 12 nm3

SASDFG9 – Poly-adenosine rA30 in 2 mM MgCl2

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 12 nm3

SASDFH9 – Poly-adenosine rA30 in 5 mM MgCl2

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFJ9 – Poly-uridine rU30 in 20 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDFK9 – Poly-uridine rU30 in 100 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 12.7 nm
VolumePorod 22 nm3

SASDFL9 – Poly-uridine rU30 in 200 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 11.0 nm
VolumePorod 15 nm3