Browse by ORGANISM: other species

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L
RgGuinier 4.3 nm
Dmax 19.5 nm
VolumePorod 58 nm3

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L
RgGuinier 4.6 nm
Dmax 22.0 nm
VolumePorod 76 nm3

SASDFB8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 78 nm3

SASDFC8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L
RgGuinier 4.5 nm
Dmax 21.0 nm
VolumePorod 74 nm3

SASDFC4 – Condensin complex subunit 3-like protein, Ycg1, extrapolated to infinite dilution

Condensin complex subunit 3-like protein experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.6 nm
Dmax 15.6 nm
VolumePorod 236 nm3

SASDFD4 – Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)

Condensin complex subunit 3-like proteinCondensin complex subunit 2 experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 2 monomer, 17 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.3 nm
Dmax 13.7 nm
VolumePorod 230 nm3

SASDFE4 – Condensin complex subunit 3-like protein, Ycg1, at 10mg/ml (tetramer)

Condensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 8.3 nm
Dmax 32.7 nm
VolumePorod 1080 nm3

SASDFF4 – Condensin complex subunit 3-like protein, Ycg1, at 5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 6.8 nm
Dmax 29.8 nm
VolumePorod 720 nm3

SASDFG4 – Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer)

Condensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 5.4 nm
Dmax 19.0 nm
VolumePorod 463 nm3

SASDFH4 – Condensin complex subunit 3-like protein, Ycg1, at 1mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 5.3 nm
Dmax 18.7 nm
VolumePorod 400 nm3