Browse by ORGANISM: other species

SASDFY7 – Aquifex aeolicus McoA metaloxidase

Aquifex aeolicus McoA metaloxidase experimental SAS data
DAMFILT model
Sample: Aquifex aeolicus McoA metaloxidase monomer, 55 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 15
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
Borges P, Brissos V, Hernandez G, Masgrau L, Lucas M, Monza E, Frazão C, Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 79 nm3

SASDH92 – Plasmodium falciparum lipocalin (PF3D7_0925900): Dimer-tetramer equilibrium through a concentration series (combined batch and SEC-SAXS measurements)

Plasmodium falciparum Lipocalin experimental SAS data
SASREF MX model
Sample: Plasmodium falciparum Lipocalin tetramer, 89 kDa Plasmodium falciparum protein
Buffer: 20 mM Tris pH7.5, 150 mM NaCl, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structure-Based Identification and Functional Characterization of a Lipocalin in the Malaria Parasite Plasmodium falciparum Cell Reports 31(12):107817 (2020)
Burda P, Crosskey T, Lauk K, Zurborg A, Söhnchen C, Liffner B, Wilcke L, Pietsch E, Strauss J, Jeffries C, Svergun D, Wilson D, Wilmanns M, Gilberger T
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 126 nm3

SASDG37 – 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
DAMMIN model
Sample: Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 dimer, 30 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM tris, 200 mM NaCl, 3% (v/v) glycerol, 0.25 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 116 nm3

SASDG47 – 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
DAMMIN model
Sample: Tegument protein UL7 dimer, 68 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 tetramer, 102 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
RgGuinier 4.6 nm
Dmax 19.7 nm
VolumePorod 340 nm3

SASDG57 – 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
GASBOR model
Sample: Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 dimer, 51 kDa Human alphaherpesvirus 1 protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
RgGuinier 4.0 nm
Dmax 18.2 nm
VolumePorod 160 nm3

SASDEX4 – Herpes simplex virus 1 tegument protein UL11

Cytoplasmic envelopment protein 3 experimental SAS data
Cytoplasmic envelopment protein 3 Kratky plot
Sample: Cytoplasmic envelopment protein 3 monomer, 12 kDa Human alphaherpesvirus 1 protein
Buffer: 50 mM HEPES, 100 mM NaCl, 0.5 mM tris(2-carboxyethyl)phosphine (TCEP),, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Jun 4
Conserved Outer Tegument Component UL11 from Herpes Simplex Virus 1 Is an Intrinsically Disordered, RNA-Binding Protein. mBio 11(3) (2020)
Metrick CM, Koenigsberg AL, Heldwein EE
RgGuinier 2.4 nm
Dmax 12.0 nm
VolumePorod 30 nm3

SASDHT4 – Phosphoprotein from Nipah virus

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein tetramer, 317 kDa Nipah henipavirus protein
Buffer: 20 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ID14-3, ESRF on 2011 Nov 3
Structural Description of the Nipah Virus Phosphoprotein and Its Interaction with STAT1. Biophys J (2020)
Jensen MR, Yabukarski F, Communie G, Condamine E, Mas C, Volchkova V, Tarbouriech N, Bourhis JM, Volchkov V, Blackledge M, Jamin M
RgGuinier 10.9 nm
Dmax 39.3 nm

SASDHS4 – Protein ninH from Bacteriophage lambda (N-terminal histidine tagged) T15A mutated

Protein ninH experimental SAS data
MODELLER model
Sample: Protein ninH dimer, 20 kDa Escherichia phage lambda protein
Buffer: 150 mM NaCl, 50 mM phosphate buffer (pH 7.4), 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 1
A bacteriophage mimic of the bacterial nucleoid-associated protein Fis. Biochem J (2020)
Chakraborti S, Balakrishnan D, Trotter AJ, Gittens WH, Yang AWH, Jolma A, Paterson JR, Świątek S, Plewka J, Curtis FA, Bowers LY, Pålsson LO, Hughes TR, Taube M, Kozak M, Heddle JG, Sharples GJ
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 33 nm3

SASDGV2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 87 nm3

SASDGW2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Nop5 (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Sep 21
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 210 nm3