Browse by ORGANISM: other species

SASDFQ9 – Poly-uridine rU30 in 2 mM MgCl2

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 16 nm3

SASDFR9 – Poly-uridine rU30 in 5 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFS9 – Poly-uridine rU30 in 10 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 10 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 14 nm3

SASDDG3 – Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with DNA

Sa0446 binding sequence 40bpTranscriptional regulator Lrs14-like protein experimental SAS data
DAMMIF model
Sample: Sa0446 binding sequence 40bp monomer, 25 kDa DNA
Transcriptional regulator Lrs14-like protein dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer: 300 mM NaCl, 20 mM HEPES, pH 7.5, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 5
Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with 40 bp DNA
Marian Vogt
RgGuinier 3.4 nm
Dmax 12.8 nm
VolumePorod 60 nm3

SASDE62 – Ribonuclease E from Yersinia pestis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 16.4 nm
VolumePorod 470 nm3

SASDE72 – Ribonuclease E from Francisella tularensis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 17.2 nm
VolumePorod 491 nm3

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 4.8 nm
Dmax 14.9 nm
VolumePorod 437 nm3

SASDE99 – Calredoxin with EGTA

Calredoxin, Redox protein from Chlamydomonas reinhardtii experimental SAS data
Calredoxin, Redox protein from Chlamydomonas reinhardtii Kratky plot
Sample: Calredoxin, Redox protein from Chlamydomonas reinhardtii monomer, 40 kDa Chlamydomonas reinhardtii protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, 5 mM EGTA, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University on 2015 Nov 17
Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga Chlamydomonas reinhardtii. J Biol Chem (2019)
Charoenwattanasatien R, Zinzius K, Scholz M, Wicke S, Tanaka H, Brandenburg JS, Marchetti GM, Ikegami T, Matsumoto T, Oda T, Sato M, Hippler M, Kurisu G
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 60 nm3

SASDEA9 – Calredoxin with Calcium

Calredoxin, Redox protein from Chlamydomonas reinhardtii experimental SAS data
Calredoxin, Redox protein from Chlamydomonas reinhardtii Kratky plot
Sample: Calredoxin, Redox protein from Chlamydomonas reinhardtii monomer, 40 kDa Chlamydomonas reinhardtii protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, 5 mM CaCl2, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University on 2015 Nov 17
Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga Chlamydomonas reinhardtii. J Biol Chem (2019)
Charoenwattanasatien R, Zinzius K, Scholz M, Wicke S, Tanaka H, Brandenburg JS, Marchetti GM, Ikegami T, Matsumoto T, Oda T, Sato M, Hippler M, Kurisu G
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 68 nm3

SASDFW4 – Conformation of R8-15 human dystrophin fragment

Human dystrophin central domain R8-15 fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Human dystrophin central domain R8-15 fragment monomer, 100 kDa protein
Buffer: NaP 10 mM, NaCl 500 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 23
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
Mias-Lucquin D, Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 10.1 nm
Dmax 36.0 nm