Small Angle Scattering Biological Data Bank SASBDB

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SASDCE5 – Light encoded DNA biosensor: e13A DNA

Sample:	E13A monomer, 17 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	24	nm³

SASDCF5 – Light encoded DNA biosensor: e13B DNA

Sample:	E13B monomer, 10 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	1.8	nm
D_max	7.0	nm
Volume^Porod	13	nm³

SASDCG5 – Light encoded DNA biosensor: e13C DNA

Sample:	E13C monomer, 6 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	1.3	nm
D_max	5.0	nm
Volume^Porod	8	nm³

SASDCH5 – Light encoded DNA biosensor: e13AB DNA

Sample:	E13AB monomer, 79 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	4.7	nm
D_max	20.0	nm
Volume^Porod	103	nm³

SASDCJ5 – Light encoded DNA biosensor: e13ABC DNA

Sample:	E13ABC monomer, 42 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	3.9	nm
D_max	18.0	nm
Volume^Porod	59	nm³

SASDCK5 – Light encoded DNA biosensor: e13Ae14Be13C DNA

Sample:	E13Ae14Be13C monomer, 40 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 16

R_g^Guinier	3.7	nm
D_max	18.0	nm
Volume^Porod	65	nm³

SASDCL5 – Light encoded DNA biosensor: e14Ae13Be14C DNA

Sample:	E14Ae13Be14C monomer, 31 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 16

R_g^Guinier	3.4	nm
D_max	14.0	nm
Volume^Porod	42	nm³

SASDDT5 – Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)

Nonstructural protein sigma NS20mer RNA (unstructured) experimental SAS data

Nonstructural protein sigma NS 20mer RNA (unstructured) Kratky plot

Sample:	Nonstructural protein sigma NS octamer, 325 kDa Avian orthoreovirus protein 20mer RNA (unstructured) dimer, 13 kDa RNA
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 25

Stability of local secondary structure determines selectivity of viral RNA chaperones. Nucleic Acids Res (2018)
Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R

R_g^Guinier	7.8	nm
D_max	38.0	nm
Volume^Porod	964	nm³

SASDDU5 – Nonstructural protein sigma NS - apoprotein

Nonstructural protein sigma NS experimental SAS data

Nonstructural protein sigma NS Kratky plot

Sample:	Nonstructural protein sigma NS hexamer, 244 kDa Avian orthoreovirus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 25

R_g^Guinier	5.5	nm
D_max	23.1	nm
Volume^Porod	670	nm³

SASDD46 – Artificially designed de novo protein esPN-Block (HL4) heterocomplex, e1s2 (HL4)

extender PN-Block (HL4)stopper PN-Block (WA20) experimental SAS data

Sample:	Extender PN-Block (HL4) monomer, 27 kDa de novo protein protein Stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein
Buffer:	20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19

Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R

R_g^Guinier	3.6	nm
D_max	15.0	nm

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