|
|
|
Sample: |
MOE PS gapmers 3-8-3 monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
|
Buffer: |
phosphate buffered saline, pH: 7.4 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2021 Oct 21
|
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
|
RgGuinier |
2.1 |
nm |
Dmax |
6.4 |
nm |
VolumePorod |
16 |
nm3 |
|
|
|
|
|
Sample: |
OME ASO monomer, 4 kDa RNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
|
Buffer: |
phosphate buffered saline, pH: 7.4 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2021 Oct 21
|
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
|
RgGuinier |
1.9 |
nm |
Dmax |
6.2 |
nm |
VolumePorod |
16 |
nm3 |
|
|
|
|
|
Sample: |
Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
|
Buffer: |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL11 - NCD, ALBA on 2019 Dec 3
|
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1.
Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
|
RgGuinier |
2.6 |
nm |
Dmax |
6.2 |
nm |
VolumePorod |
59 |
nm3 |
|
|
|
|
|
Sample: |
Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
High Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing dimer, 12 kDa DNA
|
Buffer: |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL11 - NCD, ALBA on 2019 Dec 3
|
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1.
Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
|
RgGuinier |
3.1 |
nm |
Dmax |
8.4 |
nm |
VolumePorod |
67 |
nm3 |
|
|
|
|
|
Sample: |
Protein W monomer, 15 kDa Hendra virus (isolate … protein
|
Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.2 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2023 Jun 13
|
Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.
Protein Sci 34(3):e70068 (2025)
Gondelaud F, Leval J, Arora L, Walimbe A, Bignon C, Ptchelkine D, Brocca S, Mukhopadyay S, Longhi S
|
RgGuinier |
3.4 |
nm |
Dmax |
13.5 |
nm |
VolumePorod |
42 |
nm3 |
|
|
|
|
|
Sample: |
Protein W (artificial PNT3 variant - low-kappa) monomer, 15 kDa Hendra virus (isolate … protein
|
Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.2 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2023 Jun 13
|
Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.
Protein Sci 34(3):e70068 (2025)
Gondelaud F, Leval J, Arora L, Walimbe A, Bignon C, Ptchelkine D, Brocca S, Mukhopadyay S, Longhi S
|
RgGuinier |
3.8 |
nm |
Dmax |
18.0 |
nm |
VolumePorod |
48 |
nm3 |
|
|
|
|
|
Sample: |
Protein W (artificial PNT3 variant - high-kappa) monomer, 15 kDa Hendra virus (isolate … protein
|
Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.2 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2024 Apr 5
|
Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.
Protein Sci 34(3):e70068 (2025)
Gondelaud F, Leval J, Arora L, Walimbe A, Bignon C, Ptchelkine D, Brocca S, Mukhopadyay S, Longhi S
|
RgGuinier |
3.1 |
nm |
Dmax |
14.7 |
nm |
VolumePorod |
54 |
nm3 |
|
|
|
|
|
Sample: |
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
|
Buffer: |
20 mM citrate, 150 mM NaCl, 10 mM CaCl2, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 5 |
Experiment: |
SANS
data collected at D22, Institut Laue-Langevin (ILL) on 2021 Jun 17
|
Structural characterization of pH-modulated closed and open states in a pentameric ligand-gated ion channel
Marie Lycksell
|
RgGuinier |
5.1 |
nm |
Dmax |
17.1 |
nm |
VolumePorod |
581 |
nm3 |
|
|
|
|
|
Sample: |
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
|
Buffer: |
20 mM citrate, 150 mM NaCl, 10 mM EDTA, 0.5 mM deuterated n-Dodecyl-β-D-Maltoside, in D2O, pH: 5 |
Experiment: |
SANS
data collected at D22, Institut Laue-Langevin (ILL) on 2021 Jun 17
|
Structural characterization of pH-modulated closed and open states in a pentameric ligand-gated ion channel
Marie Lycksell
|
RgGuinier |
5.2 |
nm |
Dmax |
18.4 |
nm |
VolumePorod |
558 |
nm3 |
|
|
|
|
|
Sample: |
Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
(2S)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐4‐methyl‐N‐[(pyridin‐3‐ yl)methyl]pentanamide monomer, 1 kDa
|
Buffer: |
20 mM Tris, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2021 Nov 22
|
Structure and Dynamics of Macrophage Infectivity Potentiator Proteins from Pathogenic Bacteria and Protozoans Bound to Fluorinated Pipecolic Acid Inhibitors.
J Med Chem (2025)
Pérez Carrillo VH, Whittaker JJ, Wiedemann C, Harder JM, Lohr T, Jamithireddy AK, Dajka M, Goretzki B, Joseph B, Guskov A, Harmer NJ, Holzgrabe U, Hellmich UA
|
RgGuinier |
2.9 |
nm |
Dmax |
9.7 |
nm |
VolumePorod |
61 |
nm3 |
|
|