Browse by ORGANISM: other species

SASDRM9 – Polyethylenimine-compacted single self-amplifying RNA for prophylactic and therapeutic application, specifically for vaccination

self-amplifying RNAlinear polyethylenimine experimental SAS data
DAMMIF model
Sample: Self-amplifying RNA monomer, 3030 kDa RNA
Linear polyethylenimine monomer, 25 kDa none (polymer)
Buffer: MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 10
Compact polyethylenimine-complexed mRNA molecules as quintessential vaccines
Martin Schroer
RgGuinier 12.0 nm
Dmax 40.0 nm
VolumePorod 9100 nm3

SASDSC7 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - heavy chain T219C/C224S kappa chain E123C/C214S

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - heavy chain T219C/C224SHuman immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - kappa chain E123C/C214S experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - heavy chain T219C/C224S kappa chain E123C/C214S Rg histogram
Sample: Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - heavy chain T219C/C224S dimer, 52 kDa protein
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - kappa chain E123C/C214S dimer, 47 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Apr 12
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism. Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 124 nm3

SASDW97 – Recombination directionality factor (6H-RdfS) from M.japonicum bound to 40mer DNA

Recombination directionality factor RdfSattP_8 40-mer DNA experimental SAS data
OTHER model
Sample: Recombination directionality factor RdfS tetramer, 52 kDa Mesorhizobium japonicum R7A protein
AttP_8 40-mer DNA dimer, 25 kDa DNA
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19
Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix–turn–helix protein RdfS Nucleic Acids Research 53(6) (2025)
Verdonk C, Agostino M, Eto K, Hall D, Bond C, Ramsay J
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 134 nm3

SASDW44 – Immunoglobulin G4 (pH 2, 0.2 M NaCl), SEC-SAXS data

humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) experimental SAS data
humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) Kratky plot
Sample: Humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) monomer, 148 kDa Mouse/human protein
Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Nov 19
IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state FEBS Letters (2025)
Imamura H, Honda S
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 238 nm3

SASDW54 – Immunoglobulin G1 (pH 2, 0.2 M NaCl), SEC-SAXS data

humanized immunoglobulin G1 monoclonal antibody (trastuzumab) experimental SAS data
humanized immunoglobulin G1 monoclonal antibody (trastuzumab) Kratky plot
Sample: Humanized immunoglobulin G1 monoclonal antibody (trastuzumab) monomer, 148 kDa Mouse/human protein
Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 May 20
IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state FEBS Letters (2025)
Imamura H, Honda S
RgGuinier 4.2 nm
Dmax 12.9 nm
VolumePorod 240 nm3

SASDW64 – Immunoglobulin G4 (pH 7)

humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) experimental SAS data
humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) Kratky plot
Sample: Humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) monomer, 148 kDa Mouse/human protein
Buffer: 10 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Nov 19
IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state FEBS Letters (2025)
Imamura H, Honda S
RgGuinier 4.8 nm
Dmax 15.6 nm
VolumePorod 211 nm3

SASDNX6 – ASO1 DNA in the absence of target RNA

LNA-DNA-LNA experimental SAS data
LNA-DNA-LNA Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.3 nm
Dmax 4.0 nm
VolumePorod 4 nm3

SASDNY6 – ASO2 DNA in the absence of target RNA

MOE PS gapmers 3-8-3 experimental SAS data
DAMMIF model
Sample: MOE PS gapmers 3-8-3 monomer, 4 kDa DNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 5 nm3

SASDNZ6 – ASO3 RNA in the absence of target RNA

oME ASO experimental SAS data
oME ASO Kratky plot
Sample: OME ASO monomer, 4 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.4 nm
Dmax 4.0 nm
VolumePorod 7 nm3

SASDN27 – ASO1 DNA in the presence target RNA

LNA-DNA-LNAPSCK9, 24mer ASO binding site experimental SAS data
LNA-DNA-LNA PSCK9, 24mer ASO binding site Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 15 nm3