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SASDWC2 – 2.4 mg/mL β-chitin nanofibers from squid pens with 2 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWD2 – 2.4 mg/mL β-chitin nanofibers from squid pens with 0.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWE2 – 1.2 mg/mL β-chitin nanofibers from squid pens with 1 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 72 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Acetate, 72% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWF2 – 0.48 mg/mL β-chitin nanofibers from squid pens with 1.2 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWG2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWH2 – 3 mg/mL β-chitin nanofibers from squid pens with a mixture of 1.25 mg/mL perdeuterated and 1.25 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated)GlcNAc-binding protein A experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) GlcNAc-binding protein A Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWJ2 – 3 mg/mL β-chitin nanofibers from squid pens with a mixture of 0.625 mg/mL perdeuterated and 1.875 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated)GlcNAc-binding protein A experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) GlcNAc-binding protein A Kratky plot
Sample: Β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDUS8 – HIV-1 DIS RNA with a CCCCCC apical loop (DIS-C)

HIV-1 dimerization initiation site with a CCCCCC apical loop experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering. Structure (2025)
Munsayac A, Leite WC, Hopkins JB, Hall I, O'Neill HM, Keane SC
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 11 nm3

SASDUT8 – HIV-1 DIS RNA with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension (DIS-Gk)

HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering. Structure (2025)
Munsayac A, Leite WC, Hopkins JB, Hall I, O'Neill HM, Keane SC
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 28 nm3

SASDUU8 – HIV-1 DIS-C:DIS-Gk RNA heterocomplex

HIV-1 dimerization initiation site with a CCCCCC apical loopHIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering. Structure (2025)
Munsayac A, Leite WC, Hopkins JB, Hall I, O'Neill HM, Keane SC
RgGuinier 3.5 nm
Dmax 13.5 nm
VolumePorod 40 nm3