SASDCY6 – T2 in b-DDM micelle (SEC-SAXS)

Mechanosensitive channel T2n-Dodecyl-β-D-Maltopyranoside experimental SAS data
DAMMIF model
Sample: Mechanosensitive channel T2 heptamer, 230 kDa Thermoplasma volcanium protein
n-Dodecyl-β-D-Maltopyranoside , 77 kDa synthetic construct
Buffer: 20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 May 29
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 5.6 nm
Dmax 16.5 nm
VolumePorod 896 nm3

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineDipeptide and tripeptide permease A experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine , 47 kDa synthetic construct
Dipeptide and tripeptide permease A monomer, 51 kDa Escherichia coli protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 288 nm3

SASDC27 – Saposin nanoparticle (SapNP) SapA:DOPC

saposin-a1,2-dioleoyl-sn-glycero-3-phosphocholine experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1,2-dioleoyl-sn-glycero-3-phosphocholine , 47 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDC37 – Saposin nanoparticle (SapNP) SapA:SoyPI

saposin-aL-α-phosphatidylinositol (soy) experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
L-α-phosphatidylinositol (soy) , 52 kDa Glycine max
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 3.6 nm
Dmax 8.5 nm
VolumePorod 68 nm3

SASDC47 – Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) , 46 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 10.5 nm
VolumePorod 69 nm3

SASDCA3 – Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence

Zinc finger and BTB domain-containing protein 38methylated C-terminal ZBTB38 binding sequence experimental SAS data
MONSA model
Sample: Zinc finger and BTB domain-containing protein 38 monomer, 17 kDa Homo sapiens protein
methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Nov 11
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
Pozner A, Hudson NO, Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 50 nm3

SASDCB3 – Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

methylated C-terminal ZBTB38 binding sequence experimental SAS data
DAMMIN model
Sample: methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Oct 27
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
Pozner A, Hudson NO, Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 23 nm3

SASDLM4 – Prebiotic-producing GH10 xylanase (RmXyn10A, full length)

Prebiotic-producing GH10 xylanase (RmXyn10A, full length) experimental SAS data
BUNCH model
Sample: Prebiotic-producing GH10 xylanase (RmXyn10A, full length) monomer, 108 kDa Rhodothermus marinus protein
Buffer: 50 mM TRIS, 0.5 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Mar 12
Structural insights of RmXyn10A - A prebiotic-producing GH10 xylanase with a non-conserved aglycone binding region. Biochim Biophys Acta Proteins Proteom 1866(2):292-306 (2018)
Aronsson A, Güler F, Petoukhov MV, Crennell SJ, Svergun DI, Linares-Pastén JA, Nordberg Karlsson E
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 170 nm3

SASDDQ2 – EspG3 chaperone from Mycobacterium marinum

EspG3 chaperone from Mycobacterium marinum M experimental SAS data
EspG3 chaperone from Mycobacterium marinum M Kratky plot
Sample: EspG3 chaperone from Mycobacterium marinum M monomer, 32 kDa Mycobacterium marinum M protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.3 nm
Dmax 8.0 nm

SASDDR2 – EspG1 chaperone from Mycobacterium marinum

EspG1 from Mycobacterium marinum experimental SAS data
EspG1 from Mycobacterium marinum Kratky plot
Sample: EspG1 from Mycobacterium marinum monomer, 30 kDa Mycobacterium marinum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 2.7 nm
Dmax 9.7 nm

4692 hits found.