Small Angle Scattering Biological Data Bank SASBDB

SASDNG8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer treated with PNGase F enzyme

Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data

Sample:	Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18

LARGE1 processively polymerizes length-controlled matriglycan on prodystroglycan. Nat Commun 16(1):9028 (2025)
Joseph S, Schnicker NJ, Spellmon N, Xu Z, Yan R, Yu Z, Davulcu O, Yang T, Hopkins J, Anderson ME, Venzke D, Campbell KP

R_g^Guinier	4.3	nm
D_max	18.0	nm

SASDNH8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer

Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data

Sample:	Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18

R_g^Guinier	4.2	nm
D_max	16.5	nm

SASDNJ8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer treated with PNGase F enzyme

Sample:	Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18

R_g^Guinier	4.1	nm
D_max	15.9	nm
Volume^Porod	231	nm³

SASDUS6 – Amino-terminal domain of Infectious Bursal Disease Virus VP3 at pH8.0

Structural polyprotein experimental SAS data

Sample:	Structural polyprotein dimer, 26 kDa Infectious bursal disease … protein
Buffer:	50 mM Tris, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Sep 27

Infectious Bursal Disease Virus VP3
Diego Ferrero

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	52	nm³

SASDUR7 – Infectious Bursal Disease Virus VP3 (central domains at pH 8.0)

Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) experimental SAS data

Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) Kratky plot

Sample:	Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) monomer, 18 kDa Infectious bursal disease … protein
Buffer:	50 mM TRIS, 500 mM NaCl, 2 mM DTT,, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2

Infectious Bursal Disease Virus VP3
Diego Ferrero

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	21	nm³

SASDUS7 – Infectious Bursal Disease Virus VP3 (C-terminal deleted at pH 8.0)

Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) experimental SAS data

Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) Kratky plot

Sample:	Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) dimer, 55 kDa Infectious bursal disease … protein
Buffer:	50 mM TRIS, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 1

Infectious Bursal Disease Virus VP3
Diego Ferrero

R_g^Guinier	4.1	nm
D_max	15.0	nm
Volume^Porod	110	nm³

SASDVA4 – β-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

IgA FC receptorLeukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) experimental SAS data

Sample:	IgA FC receptor monomer, 20 kDa Streptococcus agalactiae protein Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) monomer, 47 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17

Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity
Ying Zhang

R_g^Guinier	3.9	nm
D_max	13.5	nm
Volume^Porod	202	nm³

SASDVB4 – β-Antigen C Protein B75KN-CTD domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)IgA FC receptor (N770H) experimental SAS data

Sample:	Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R) monomer, 47 kDa Homo sapiens protein IgA FC receptor (N770H) monomer, 12 kDa Streptococcus agalactiae protein
Buffer:	20 mM HEPES, 300 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17

Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity
Ying Zhang

R_g^Guinier	3.9	nm
D_max	14.1	nm
Volume^Porod	175	nm³

SASDXF8 – β-Antigen C Protein bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

IgA FC receptor β-Antigen C ProteinLeukocyte immunoglobulin-like receptor subfamily B3 experimental SAS data

Sample:	IgA FC receptor β-Antigen C Protein monomer, 71 kDa Streptococcus agalactiae protein Leukocyte immunoglobulin-like receptor subfamily B3 monomer, 94 kDa Homo sapiens protein
Buffer:	20mM HEPES, 140mM NaCl, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Oct 5

Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity
Ying Zhang

R_g^Guinier	7.0	nm
D_max	26.5	nm
Volume^Porod	447	nm³

SASDXG8 – Streptococcus agalactiae IgA FC receptor

IgA FC receptor β-Antigen C Protein experimental SAS data

Sample:	IgA FC receptor β-Antigen C Protein monomer, 71 kDa Streptococcus agalactiae protein
Buffer:	20mM HEPES, 140mM NaCl, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Oct 5

Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity

R_g^Guinier	5.9	nm
D_max	20.2	nm
Volume^Porod	140	nm³

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