Small Angle Scattering Biological Data Bank SASBDB


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18 hits found for Dmitri Svergun

SASDC52 – Bruton tyrosine kinase

Tyrosine-protein kinase BTK (R28C mutant) experimental SAS data

Sample:	Tyrosine-protein kinase BTK (R28C mutant) monomer, 76 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl, 2 mM DTT and 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2002 Apr 2

Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering. EMBO J 22(18):4616-24 (2003)
...Svergun DI

R_g^Guinier	5.0	nm
D_max	20.0	nm
Volume^Porod	130	nm³

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data

Sample:	Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Svergun DI

R_g^Guinier	2.8	nm
D_max	8.2	nm
Volume^Porod	100	nm³

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data

Sample:	Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Svergun DI

R_g^Guinier	3.9	nm
D_max	12.7	nm
Volume^Porod	202	nm³

SASDJF4 – Synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23 experimental SAS data

Sample:	Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Buffer:	50 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 5

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 Nature Communications 11(1) (2020)
...Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C

R_g^Guinier	2.1	nm
D_max	8.0	nm
Volume^Porod	22	nm³

SASDJG4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD)

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	25 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 1

R_g^Guinier	3.0	nm
D_max	13.1	nm
Volume^Porod	64	nm³

SASDJH4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) bound to the synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Sample:	Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	25 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 10

R_g^Guinier	3.5	nm
D_max	15.1	nm
Volume^Porod	87	nm³

SASDAK5 – Myomesin-1 My12-My13

Sample:	Myomesin-1 dimer, 46 kDa Homo sapiens protein
Buffer:	25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 7

Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin. EMBO J 27(1):253-64 (2008)
...Svergun DI, Wilmanns M

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	56	nm³

SASDA96 – Lysozyme

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	20 mM Sodium Acetate/HEPES, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Feb 17

Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
...Svergun DI

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	24	nm³

SASDAA6 – Human serum albumin monomer and mixtures

Human serum albumin monomer experimental SAS data

Sample:	Human serum albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22

Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
...Svergun DI

R_g^Guinier	2.8	nm
D_max	8.4	nm
Volume^Porod	103	nm³

SASDAB6 – Xylose Isomerase

Sample:	Xylose Isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	20 mM HEPES 200 mM Na2SO4 50 mM K2SO4 500 % v/v D2O 1 mM MgCl2, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 10

Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
...Svergun DI

R_g^Guinier	3.2	nm
D_max	9.1	nm
Volume^Porod	234	nm³