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11 hits found for Saharan

SASDUK2 – Histone deacetylase 7

Histone deacetylase 7 experimental SAS data
COOT model
Sample: Histone deacetylase 7 monomer, 44 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 24
Structure-function analyses reveal Arabidopsis thaliana HDA7 to be an inactive histone deacetylase Current Research in Structural Biology :100136 (2024)
Saharan K, Baral S, Shaikh N, Vasudevan D
RgGuinier 3.0 nm
Dmax 10.2 nm
VolumePorod 85 nm3

SASDVQ2 – Nucleoplasmin-like domain-containing protein PfNPM (1-98)

Nucleoplasmin-like domain-containing protein experimental SAS data
DAMMIF model
Sample: Nucleoplasmin-like domain-containing protein pentamer, 60 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM tris, 150 mM NaCl and 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 28
Plasmodium falciparum nucleoplasmin (PfNPM)
Ketul Saharan
RgGuinier 2.4 nm
Dmax 7.0 nm
VolumePorod 98 nm3

SASDVR2 – Nucleoplasmin-like domain-containing protein PfNPM (1-110)

Nucleoplasmin-like domain-containing protein A1 experimental SAS data
DAMMIF model
Sample: Nucleoplasmin-like domain-containing protein A1 pentamer, 67 kDa Plasmodium falciparum 3D7 protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 28
Plasmodium falciparum nucleoplasmin (PfNPM)
Ketul Saharan
RgGuinier 2.8 nm
Dmax 8.6 nm
VolumePorod 141 nm3

SASDVS2 – Nucleoplasmin-like domain-containing protein PfNPM (1-170)

N-terminal domain of Nucleoplasmin-like domain-containing protein A3 experimental SAS data
DAMMIF model
Sample: N-terminal domain of Nucleoplasmin-like domain-containing protein A3 pentamer, 103 kDa Plasmodium falciparum 3D7 protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 28
Plasmodium falciparum nucleoplasmin (PfNPM)
Ketul Saharan
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 204 nm3

SASDVT2 – Nucleoplasmin-like domain-containing protein PfNPM (1-170) - H2A/H2B histone oligomer complex

PfNPM A3 (1-170)Histone H2A type 1-B/EHistone H2B type 1-C/E/F/G/I experimental SAS data
DAMMIF model
Sample: PfNPM A3 (1-170) pentamer, 103 kDa Plasmodium falciparum 3D7 protein
Histone H2A type 1-B/E monomer, 14 kDa Homo sapiens protein
Histone H2B type 1-C/E/F/G/I monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 28
Plasmodium falciparum nucleoplasmin (PfNPM)
Ketul Saharan
RgGuinier 4.5 nm
Dmax 13.9 nm
VolumePorod 229 nm3

SASDVU2 – Nucleoplasmin-like domain-containing protein PfNPM (1-170) - H3/H4 histone oligomer complex

Histone H3.1PfNPM A3 (1-170)Histone H4 experimental SAS data
DAMMIN model
Sample: Histone H3.1 dimer, 31 kDa Homo sapiens protein
PfNPM A3 (1-170) pentamer, 103 kDa Plasmodium falciparum 3D7 protein
Histone H4 dimer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 28
Plasmodium falciparum nucleoplasmin (PfNPM)
Ketul Saharan
RgGuinier 4.9 nm
Dmax 13.8 nm
VolumePorod 284 nm3

SASDNN3 – Peptidyl-prolyl cis-trans isomerase FKBP43, nucleoplasmin domain (amino acids 1-96)

Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 58 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 16
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
...Saharan K, Baral S, Vasudevan D
RgGuinier 2.5 nm
Dmax 7.3 nm
VolumePorod 141 nm3

SASDNQ7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136)

Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
...Saharan K, Baral S, Vasudevan D
RgGuinier 3.6 nm
Dmax 12.4 nm
VolumePorod 164 nm3

SASDNT7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-164)

Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 96 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
...Saharan K, Baral S, Vasudevan D
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 180 nm3

SASDNR7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H2A/H2B histone oligomer complex

Peptidyl-prolyl cis-trans isomerase FKBP43Histone H2A type 1-B/EHistone H2B type 1-C/E/F/G/I experimental SAS data
MULTIFOXS model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Histone H2A type 1-B/E monomer, 14 kDa Homo sapiens protein
Histone H2B type 1-C/E/F/G/I monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
...Saharan K, Baral S, Vasudevan D
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 194 nm3

SASDNS7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H3/H4 histone oligomer complex

Peptidyl-prolyl cis-trans isomerase FKBP43Histone H3.1Histone H4 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Histone H3.1 dimer, 31 kDa Homo sapiens protein
Histone H4 dimer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
...Saharan K, Baral S, Vasudevan D
RgGuinier 4.4 nm
Dmax 14.1 nm
VolumePorod 226 nm3