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4 hits found for Zuzanna Pietras

SASDP34 – Ubiquitin-conjugating enzyme E2 conjugated to deuterated Ubiquitin (SANS data at 0% v/v D2O, plus additional SANS with contrast variation and SAXS datasets)

Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S)Ubiquitin experimental SAS data
MONSA model
Sample: Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S) monomer, 17 kDa Homo sapiens protein
Ubiquitin monomer, 9 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2019 May 24
Production and characterisation of modularly deuterated UBE2D1–Ub conjugate by small angle neutron and X-ray scattering European Biophysics Journal (2022)
Pietras Z, Duff A, Morad V, Wood K, Jeffries C, Sunnerhagen M
RgGuinier 2.1 nm
Dmax 7.4 nm

SASDWE4 – The nonstructural 2A protein of the human parechovirus 1 (HPeV1) short form, amino acids 1-129

Genome polyprotein (Protein 2A H-NC; Δ130-150) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Genome polyprotein (Protein 2A H-NC; Δ130-150) , 15 kDa Human parechovirus 1 … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM DDT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 28
Structural plasticity of 2A proteins in the Parechovirus family
Zuzanna Pietras
RgGuinier 2.0 nm
Dmax 9.5 nm
VolumePorod 43 nm3

SASDMG9 – Multidrug resistance operon repressor (MexR) of the MexAB-OprM multidrug efflux pump operon of Pseudomonas aeruginosa

Multidrug resistance operon repressor experimental SAS data
DAMFILT model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
Buffer: 20mM HEPES, 150mM NaCl, 10mM DTT, 1% v/v glycerol, pH: 7.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 23
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
...Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 56 nm3

SASDMH9 – Pseudomonas aeruginosa Multidrug resistance operon repressor (MexR) in complex with 34bp dsDNA binding sequence (SANS data at 0% D2O)

Multidrug resistance operon repressor34 base pair double-stranded DNA experimental SAS data
MONSA model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
34 base pair double-stranded DNA monomer, 21 kDa synthetic construct DNA
Buffer: 20mM NaPO4, 150 mM NaCl, 10 mM DTT, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 May 30
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
...Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.9 nm
Dmax 7.8 nm
VolumePorod 79 nm3