Small Angle Scattering Biological Data Bank SASBDB


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5 hits found for DNA operator

SASDPK3 – ...DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein ...DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – ...DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein ...DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – ...DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein ...DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDS86 – ...DNA operator sequence

Antitoxin HigA-2DNA operator experimental SAS data

Antitoxin HigA-2 DNA operator Kratky plot

Sample:	Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein DNA operator dimer, 20 kDa DNA
Buffer:	20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Apr 6

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat Commun 15(1):3105 (2024)
Hadži S, Živič Z, Kovačič M, Zavrtanik U, Haeserts S, Charlier D, Plavec J, Volkov AN, Lah J, Loris R

R_g^Guinier	2.7	nm
D_max	9.6	nm
Volume^Porod	57	nm³

SASDE68 – ...operator

GraTA operator regionantitoxin GraA, antidote of toxin GraTatitoxin GraA, antidote of GraT. experimental SAS data

GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot

Sample:	...operator region monomer, 20 kDa DNA antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein atitoxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Dec 17

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R

R_g^Guinier	2.9	nm
D_max	10.3	nm
Volume^Porod	94	nm³

Search

SASDPK3 – ...DNA operator box

SASDPL3 – ...DNA operator box

SASDPM3 – ...DNA operator fragment

SASDS86 – ...DNA operator sequence

SASDE68 – ...operator