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8 hits found for glucan 1,3-beta-glucosidase

SASDVL8 – Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated monomer

glucan 1,3-beta-glucosidase experimental SAS data
ALPHAFOLD model
Sample: glucan 1,3-beta-glucosidase monomer, 45 kDa Mucor circinelloides protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2020 Mar 26
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.3 nm
Dmax 6.4 nm
VolumePorod 53 nm3

SASDVM8 – Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated dimer

glucan 1,3-beta-glucosidase experimental SAS data
Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated dimer Rg histogram
Sample: glucan 1,3-beta-glucosidase dimer, 92 kDa Mucor circinelloides protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 27
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 140 nm3

SASDVN8 – Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated dimer

glucan 1,3-beta-glucosidase (short form, glycosylated) experimental SAS data
DAMMIN model
Sample: glucan 1,3-beta-glucosidase (short form, glycosylated) dimer, 89 kDa Mucor circinelloides protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 28
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 3.3 nm
Dmax 11.0 nm
VolumePorod 103 nm3

SASDVP8 – Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated tetramer

glucan 1,3-beta-glucosidase (long form, glycosylated) experimental SAS data
Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated tetramer Rg histogram
Sample: glucan 1,3-beta-glucosidase (long form, glycosylated) tetramer, 184 kDa Mucor circinelloides protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 28
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 4.8 nm
Dmax 15.1 nm
VolumePorod 171 nm3

SASDVQ8 – Glucosidase/Rutinosidase from Penicillium rubens - deglycosylated

glucan 1,3-beta-glucosidase experimental SAS data
ALPHAFOLD model
Sample: glucan 1,3-beta-glucosidase monomer, 42 kDa Penicillium rubens (strain … protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Nov 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.1 nm
Dmax 5.5 nm
VolumePorod 65 nm3

SASDVR8 – Glucosidase/Rutinosidase from Penicillium rubens - glycosylated

glucan 1,3-beta-glucosidase experimental SAS data
ALPHAFOLD model
Sample: glucan 1,3-beta-glucosidase monomer, 42 kDa Penicillium rubens (strain … protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Nov 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 68 nm3

SASDVS8 – Glucosidase/Rutinosidase from Aspergillus niger - deglycosylated

glucan 1,3-beta-glucosidase (W90L, N179H) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: glucan 1,3-beta-glucosidase (W90L, N179H) monomer, 42 kDa Aspergillus niger (strain … protein
Buffer: McIlvain (Na2HPO4/citric acid) buffer, pH: 3.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Jun 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.2 nm
Dmax 6.1 nm
VolumePorod 55 nm3

SASDVT8 – Glucosidase/Rutinosidase from Aspergillus niger - glycosylated

glucan 1,3-beta-glucosidase (W90L, N179H) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: glucan 1,3-beta-glucosidase (W90L, N179H) dimer, 83 kDa Aspergillus niger (strain … protein
Buffer: McIlvain (Na2HPO4/citric acid) buffer, pH: 3.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Jun 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 3.0 nm
Dmax 8.1 nm
VolumePorod 111 nm3