Search

  
Advanced search  

157 hits found for idp

SASDTQ6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.45 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) , 16 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 22.3 nm

SASDTR6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.66 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) , 16 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 17.8 nm

SASDTS6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1.1 mg/ml - 20 mM Tris pH 8.0

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.3 nm

SASDET6 – Polyglutamine-binding protein 1 p.Lys192Serfs*7 (PQBP-1 XLID mutant K192Sfs*7)

Polyglutamine-binding protein 1 p.Lys192Serfs*7 experimental SAS data
Polyglutamine-binding protein 1 p.Lys192Serfs*7 (PQBP-1 XLID mutant K192Sfs*7) Rg histogram
Sample: Polyglutamine-binding protein 1 p.Lys192Serfs*7 dimer, 47 kDa Homo sapiens protein
Buffer: Phosphate-buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2013 Feb 15
Frameshift PQBP-1 mutants K192Sfs*7 and R153Sfs*41 implicated in X-linked intellectual disability form stable dimers. J Struct Biol (2019)
Rahman SK, Okazawa H, Chen YW
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 114 nm3

SASDTT6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.8 mg/ml - 20 mM Tris pH 8.0

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.4 nm

SASDEU6 – Polyglutamine-binding protein 1 p.Arg153Serfs*41 (PQBP-1 XLID mutant R153Sfs*41)

Polyglutamine-binding protein 1 p.Arg153Serfs*41 experimental SAS data
Polyglutamine-binding protein 1 p.Arg153Serfs*41 (PQBP-1 XLID mutant R153Sfs*41) Rg histogram
Sample: Polyglutamine-binding protein 1 p.Arg153Serfs*41 dimer, 44 kDa Homo sapiens protein
Buffer: Phosphate-buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2013 Feb 15
Frameshift PQBP-1 mutants K192Sfs*7 and R153Sfs*41 implicated in X-linked intellectual disability form stable dimers. J Struct Biol (2019)
Rahman SK, Okazawa H, Chen YW
RgGuinier 3.6 nm
Dmax 13.0 nm
VolumePorod 100 nm3

SASDTU6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.6 mg/ml - 20 mM Tris pH 8.0

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.7 nm

SASDTV6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1 mg/ml - 20 mM Tris pH 8.0, 50 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 50 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.2 nm

SASDTW6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.5 mg/ml - 20 mM Tris pH 8.0, 50 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 50 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.6 nm

SASDTX6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 50 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 50 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.2 nm

SASDTY6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1.5 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.3 nm

SASDBZ6 – Draxin

Draxin experimental SAS data
Draxin Rg histogram
Sample: Draxin monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 87 nm3

SASDTZ6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.8 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.7 nm

SASDF27 – Brain and Muscle ARNT-Like 1 from Mus musculus (D530-L625), monomer, trans-conformation locking mutation P624A

Aryl hydrocarbon receptor nuclear translocator-like protein 1 experimental SAS data
DAMMIN model
Sample: Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein
Buffer: 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27
Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein. J Biol Chem (2019)
Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E
RgGuinier 2.8 nm
Dmax 11.0 nm

SASDT27 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.5 nm

SASDT37 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.6 nm

SASDU37 – The intrinsically disordered region of NAC domain-containing protein 13 (ANAC013; amino acids 161-274)

NAC domain-containing protein 13 experimental SAS data
NAC domain-containing protein 13 Kratky plot
Sample: NAC domain-containing protein 13 monomer, 13 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Allovalent scavenging of activation domains in the transcription factor ANAC013 gears transcriptional regulation. Nucleic Acids Res 53(4) (2025)
Delaforge E, Due AD, Theisen FF, Morffy N, O'Shea C, Blackledge M, Strader LC, Skriver K, Kragelund BB
RgGuinier 3.6 nm
Dmax 16.0 nm
VolumePorod 36 nm3

SASDT47 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.5 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.6 nm

SASDT57 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.4 nm

SASDT67 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1.5 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.0 nm

SASDU67 – The C-terminal region of DNA repair protein XRCC4

DNA repair protein XRCC4 experimental SAS data
DNA repair protein XRCC4 Kratky plot
Sample: DNA repair protein XRCC4 monomer, 15 kDa Homo sapiens protein
Buffer: 20 mM Bis-tris, 150 mM KCl, 1 mM EDTA, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 20
Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro. Nat Struct Mol Biol (2024)
Vu DD, Bonucci A, Brenière M, Cisneros-Aguirre M, Pelupessy P, Wang Z, Carlier L, Bouvignies G, Cortes P, Aggarwal AK, Blackledge M, Gueroui Z, Belle V, Stark JM, Modesti M, Ferrage F
RgGuinier 3.4 nm
Dmax 11.1 nm
VolumePorod 41 nm3

SASDT77 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.78 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.0 nm

SASDT87 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.38 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 4.2 nm

SASDQB7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 3.7 nm
Dmax 19.0 nm
VolumePorod 48 nm3

SASDQC7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region) mutated YYY --> AAA

Non-structural protein V (Y211A, Y212A, Y213A mutant) experimental SAS data
Non-structural protein V (Y211A, Y212A, Y213A mutant) Kratky plot
Sample: Non-structural protein V (Y211A, Y212A, Y213A mutant) monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 4.0 nm
Dmax 19.0 nm
VolumePorod 49 nm3

SASDQD7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region, C-terminal truncated)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 9 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 2.8 nm
Dmax 13.0 nm
VolumePorod 22 nm3

SASDQE7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region C-terminal truncated), mutated YYY --> AAA

Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) experimental SAS data
Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) Kratky plot
Sample: Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) monomer, 9 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 2.7 nm
Dmax 12.5 nm
VolumePorod 21 nm3

SASDQF7 – Region within N-terminal domain of P/V/W protein of Nipah virus (PNT3 region)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 15 kDa Nipah henipavirus protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 3.7 nm
Dmax 16.5 nm
VolumePorod 47 nm3

SASDJS7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
Ostendorp A, Ostendorp S, Zhou Y, Chaudron Z, Wolffram L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 46 nm3

SASDJT7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
Ostendorp A, Ostendorp S, Zhou Y, Chaudron Z, Wolffram L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 48 nm3

SASDK68 – Transcriptional intermediary factor 2 (TIF2) intrinsically disordered coactivator

Nuclear receptor coactivator 2 experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Nuclear receptor coactivator 2 monomer, 16 kDa Homo sapiens protein
Buffer: 150 mM NaCl, 50 mM BisTris pH 6.8, and 0.5 mM EDTA, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 14
Structural Insights into the Interaction of the Intrinsically Disordered Co-activator TIF2 with Retinoic Acid Receptor Heterodimer (RXR/RAR). J Mol Biol 433(9):166899 (2021)
Senicourt L, le Maire A, Allemand F, Carvalho JE, Guee L, Germain P, Schubert M, Bernadó P, Bourguet W, Sibille N
RgGuinier 3.7 nm
Dmax 16.3 nm
VolumePorod 50 nm3

SASDP88 – N-terminal domain of S. cerevisiae eukaryotic initiation factor 4F subunit p150, eIF4G1 (Tif4631)

Eukaryotic initiation factor 4F subunit p150 experimental SAS data
Eukaryotic initiation factor 4F subunit p150 Kratky plot
Sample: Eukaryotic initiation factor 4F subunit p150 , 28 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM potassium phosphate, 25 mM NaCl, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14
eIF4G1 N-terminal intrinsically disordered domain is a multi-docking station for RNA, Pab1, Pub1, and self-assembly. Front Mol Biosci 9:986121 (2022)
Chaves-Arquero B, Martínez-Lumbreras S, Sibille N, Camero S, Bernadó P, Jiménez MÁ, Zorrilla S, Pérez-Cañadillas JM
RgGuinier 5.2 nm
Dmax 19.0 nm
VolumePorod 137 nm3

SASDHF8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K Rg histogram
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13
Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.4 nm
Dmax 6.0 nm
VolumePorod 3 nm3

SASDHG8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 2.51 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin-3, His3-(20-43)-peptide Kratky plot
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13
Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.5 nm
Dmax 6.4 nm
VolumePorod 3 nm3

SASDHH8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 1.26 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin-3, His3-(20-43)-peptide Kratky plot
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13
Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.5 nm
Dmax 6.0 nm
VolumePorod 3 nm3

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data
EKC/KEOPS complex subunit GON7 Kratky plot
Sample: EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 26
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, Liger D, Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC,...
RgGuinier 3.1 nm
Dmax 12.5 nm
VolumePorod 46 nm3

SASDQM8 – G. gallus TRPV4 intrinsically disordered N-terminus

Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 intrinsically disordered N-terminus Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 15 kDa Gallus gallus protein
Buffer: 20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 3.2 nm
Dmax 14.5 nm
VolumePorod 59 nm3

SASDQN8 – G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111

Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111 Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 14 kDa Gallus gallus protein
Buffer: 20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 3.3 nm
Dmax 14.5 nm
VolumePorod 37 nm3

SASDKT8 – von Willebrand Factor peptide 3mg/ml

von Willebrand factor experimental SAS data
von Willebrand factor Kratky plot
Sample: von Willebrand factor monomer, 11 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24
An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution. J Mol Biol 433(13):166954 (2021)
Del Amo-Maestro L, Sagar A, Pompach P, Goulas T, Scavenius C, Ferrero DS, Castrillo-Briceño M, Taulés M, Enghild JJ, Bernadó P, Gomis-Rüth FX
RgGuinier 3.1 nm
Dmax 14.0 nm
VolumePorod 28 nm3

SASDKU8 – von Willebrand Factor peptide 6mg/ml

von Willebrand factor experimental SAS data
von Willebrand Factor peptide 6mg/ml Rg histogram
Sample: von Willebrand factor monomer, 11 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24
An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution. J Mol Biol 433(13):166954 (2021)
Del Amo-Maestro L, Sagar A, Pompach P, Goulas T, Scavenius C, Ferrero DS, Castrillo-Briceño M, Taulés M, Enghild JJ, Bernadó P, Gomis-Rüth FX
RgGuinier 3.0 nm
Dmax 14.9 nm
VolumePorod 27 nm3

SASDK29 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 1.3 mg/ml

Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2 , 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 3.8 nm
Dmax 21.0 nm

SASDL69 – Ectodomain of human syndecan-1

Syndecan-1 experimental SAS data
Ectodomain of human syndecan-1 Rg histogram
Sample: Syndecan-1 monomer, 27 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Sep 26
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 5.3 nm

SASDL79 – Ectodomain of human syndecan-2

Syndecan-2 experimental SAS data
Ectodomain of human syndecan-2 Rg histogram
Sample: Syndecan-2 monomer, 17 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 31
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 4.3 nm

SASDL89 – Ectodomain of human syndecan-3

Syndecan-3 experimental SAS data
Ectodomain of human syndecan-3 Rg histogram
Sample: Syndecan-3 monomer, 39 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 May 29
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 6.5 nm

SASDL99 – Ectodomain of human syndecan-4 isoform-2 Monomer

Syndecan-4 experimental SAS data
Ectodomain of human syndecan-4 isoform-2 Monomer Rg histogram
Sample: Syndecan-4 monomer, 18 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Jul 4
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 4.2 nm

SASDKC9 – Mothers against decapentaplegic homolog 4, SMAD4, wild-type

Mothers against decapentaplegic homolog 4 experimental SAS data
Mothers against decapentaplegic homolog 4 Kratky plot
Sample: Mothers against decapentaplegic homolog 4 monomer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 4
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 4.4 nm
Dmax 22.0 nm

SASDKE9 – Mothers against decapentaplegic homolog 4, SMAD4, linker-MH2 fragment (amino acids 150-552)

Mothers against decapentaplegic homolog 4 (linker-MH2) experimental SAS data
Mothers against decapentaplegic homolog 4 (linker-MH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (linker-MH2) monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 3.3 nm
Dmax 17.0 nm
VolumePorod 80 nm3

SASDKF9 – Mothers against decapentaplegic homolog 4, SMAD4, SADMH2 fragment (amino acids 272-552)

Mothers against decapentaplegic homolog 4 (SADMH2) experimental SAS data
Mothers against decapentaplegic homolog 4 (SADMH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (SADMH2) monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 54 nm3

SASDLF9 – 200-310 region of Hendra virus P/V/W protein (PNT3)

Protein W experimental SAS data
200-310 region of Hendra virus P/V/W protein (PNT3) Rg histogram
Sample: Protein W monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, 5 mM EDTA, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation Biomolecules 11(9):1324 (2021)
Salladini E, Gondelaud F, Nilsson J, Pesce G, Bignon C, Murrali M, Fabre R, Pierattelli R, Kajava A, Horvat B, Gerlier D, Mathieu C, Longhi S
RgGuinier 3.4 nm
Dmax 15.5 nm
VolumePorod 38 nm3

SASDKG9 – Mothers against decapentaplegic homolog 4, SMAD4, MH2 fragment (amino acids 314-552)

Mothers against decapentaplegic homolog 4 (MH2 Fragment) experimental SAS data
Mothers against decapentaplegic homolog 4 (MH2 Fragment) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (MH2 Fragment) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 2.1 nm
Dmax 8.0 nm

SASDLK9 – Hendra virus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Hendra virus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins International Journal of Molecular Sciences 23(2):923 (2022)
Pesce G, Gondelaud F, Ptchelkine D, Nilsson J, Bignon C, Cartalas J, Fourquet P, Longhi S
RgGuinier 7.2 nm
Dmax 24.0 nm
VolumePorod 338 nm3

SASDLL9 – Nipah henipavirus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Nipah henipavirus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Nipah henipavirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins International Journal of Molecular Sciences 23(2):923 (2022)
Pesce G, Gondelaud F, Ptchelkine D, Nilsson J, Bignon C, Cartalas J, Fourquet P, Longhi S
RgGuinier 7.1 nm
Dmax 24.5 nm
VolumePorod 327 nm3

SASDHV9 – Murine major prion protein PrPc 23-230

Major prion protein experimental SAS data
CORAL model
Sample: Major prion protein monomer, 23 kDa Mus musculus protein
Buffer: 10mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Characterization of murine prion protein
Stefano Da Vela
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 43 nm3

SASDCY9 – Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii

Calvin cycle protein CP12, chloroplastic experimental SAS data
Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Buffer: 50 mM phosphate buffer, 50 mM NaCl, 20 mM oxidized DTT, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 3
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 22 nm3

SASDKD6 – NURS complex subunit red1

NURS complex subunit red1 experimental SAS data
NURS complex subunit red1 Kratky plot
Sample: NURS complex subunit red1 monomer, 8 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex. Nat Commun 14(1):772 (2023)
Soni K, Sivadas A, Horvath A, Dobrev N, Hayashi R, Kiss L, Simon B, Wild K, Sinning I, Fischer T
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 12 nm3

SASDKH8 – C-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor

Translocated intimin receptor Tir experimental SAS data
Translocated intimin receptor Tir Kratky plot
Sample: Translocated intimin receptor Tir monomer, 18 kDa Escherichia coli O127:H6 … protein
Buffer: 20 mM Sodium Phosphate, 150 mM NaCl, 1 mM EDTA, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 15
The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection. Commun Biol 7(1):179 (2024)
Vieira MFM, Hernandez G, Zhong Q, Arbesú M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazão C, Frankel G, Zanzoni A, Cordeiro TN
RgGuinier 3.8 nm
Dmax 12.8 nm
VolumePorod 46 nm3

SASDUX9 – Br-labelled Elastin-like Polypeptide (ELP) in 6 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.8 nm
Dmax 18.1 nm
VolumePorod 47 nm3