SASBDB entries for UniProt ID:

SASDHH5 – Nuclear factor kB p50/RelA bound to Urokinase kB site DNA

UniProt ID: Q04207 (19-549) Transcription factor p65 19-549

UniProt ID: P25799 (39-350) NF-kappa-B p105 subunit 39-350

UniProt ID: None (None-None) Urokinase kB
Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350Urokinase kB experimental SAS data
Transcription factor p65 19-549 NF-kappa-B p105 subunit 39-350 Urokinase kB Kratky plot
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Urokinase kB monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.7 nm
Dmax 17.5 nm
VolumePorod 201 nm3

SASDHJ5 – Apolipoprotein-D plus biliverdin

UniProt ID: P05090 (21-189) Apolipoprotein D
Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 168 nm3

SASDHK5 – Apolipoprotein-D oxidised with H2O2

UniProt ID: P05090 (21-189) Apolipoprotein D
Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.3 nm
VolumePorod 164 nm3

SASDHL5 – Apolipoprotein-D plus palmitic acid

UniProt ID: P05090 (21-189) Apolipoprotein D
Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.6 nm
VolumePorod 163 nm3

SASDHM5 – Apolipoprotein-D plus progesterone

UniProt ID: P05090 (21-189) Apolipoprotein D
Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.0 nm
VolumePorod 161 nm3

SASDHN5 – Apolipoprotein-D plus sphingomyelin

UniProt ID: P05090 (21-189) Apolipoprotein D
Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 9.9 nm
VolumePorod 171 nm3

SASDHP5 – Rov C - Regulator Of Virulence interconnected with the Csr system (hexameric transcriptional activator of type VI secretion gene expression)

UniProt ID: A0A0H3B5N9 (1-247) Regulator Of Virulence interconnected with the Csr system
Regulator Of Virulence interconnected with the Csr system experimental SAS data
CORAL model
Sample: Regulator Of Virulence interconnected with the Csr system hexamer, 175 kDa Yersinia pseudotuberculosis protein
Buffer: 50 mM TRIS pH= 8, 500 mM NaCl, 5 mM DTT, 5 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 18
RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression PLOS Pathogens 16(9):e1008552 (2020)
Knittel V, Sadana P, Seekircher S, Stolle A, Körner B, Volk M, Jeffries C, Svergun D, Heroven A, Scrima A, Dersch P, Mecsas J
RgGuinier 4.2 nm
Dmax 12.4 nm
VolumePorod 280 nm3

SASDHS5 – Full-length Mouse TIFA dimer

UniProt ID: Q793I8 (1-184) TRAF-interacting protein with FHA domain-containing protein A
TRAF-interacting protein with FHA domain-containing protein A experimental SAS data
DAMFILT model
Sample: TRAF-interacting protein with FHA domain-containing protein A dimer, 45 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 100 mM arginine, 5 % glycerol, 10 mM DTT, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Kumamoto University on 2014 Oct 16
Structural analysis of TIFA: Insight into TIFA-dependent signal transduction in innate immunity. Sci Rep 10(1):5152 (2020)
Nakamura T, Hashikawa C, Okabe K, Yokote Y, Chirifu M, Toma-Fukai S, Nakamura N, Matsuo M, Kamikariya M, Okamoto Y, Gohda J, Akiyama T, Semba K, Ikemizu S, Otsuka M, Inoue JI, Yamagata Y
RgGuinier 3.1 nm
Dmax 15.5 nm
VolumePorod 85 nm3

SASDHT5 – Human Carbonic Anhydrase IX

UniProt ID: Q16790 (38-414) Carbonic anhydrase 9
Carbonic anhydrase 9 experimental SAS data
Human Carbonic Anhydrase IX Rg histogram
Sample: Carbonic anhydrase 9 monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Nov 16
Biophysical Characterization of Cancer-Related Carbonic Anhydrase IX. Int J Mol Sci 21(15) (2020)
Koruza K, Murray AB, Mahon BP, Hopkins JB, Knecht W, McKenna R, Fisher SZ
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 71 nm3

SASDHV5 – Human 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) at 1.25 mg/mL

UniProt ID: P49189 (2-494) 4-trimethylaminobutyraldehyde dehydrogenase
4-trimethylaminobutyraldehyde dehydrogenase experimental SAS data
OTHER model
Sample: 4-trimethylaminobutyraldehyde dehydrogenase tetramer, 215 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 600 mM NaCl, 2% (v/v) glycerol, 1 mM DTT, 1 mM NAD+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 28
Inhibition, crystal structures, and in-solution oligomeric structure of aldehyde dehydrogenase 9A1. Arch Biochem Biophys :108477 (2020)
Wyatt JW, Korasick DA, Qureshi IA, Campbell AC, Gates KS, Tanner JJ
RgGuinier 3.8 nm
Dmax 10.9 nm
VolumePorod 252 nm3