SASBDB entries for UniProt ID:

SASDJN4 – ATP-dependent DNA helicase UvrD-ATP complex

UniProt ID: P9WMQ1 (1-771) ATP-dependent DNA helicase UvrD1
ATP-dependent DNA helicase UvrD1 experimental SAS data
DAMFILT model
Sample: ATP-dependent DNA helicase UvrD1 monomer, 85 kDa Mycobacterium tuberculosis protein
Buffer: 10 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2, 0.1 mM EDTA, 5% Glycerol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Feb 28
Structural and biochemical characterization of RNA polymerase core and UvrD complex: a key component in transcription coupled DNA repair
Ravishankar Ramachandran
RgGuinier 3.5 nm
Dmax 9.5 nm
VolumePorod 101 nm3

SASDJR4 – Type 3 secretion system pilotin, InvH27-147

UniProt ID: P0CL43 (27-147) Type 3 secretion system pilotin
Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 28 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods. Structure (2020)
Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ
RgGuinier 3.1 nm
Dmax 12.6 nm
VolumePorod 53 nm3

SASDJS4 – Type 3 secretion system pilotin, InvH70-147

UniProt ID: P0CL43 (70-147) Type 3 secretion system pilotin
Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods. Structure (2020)
Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 29 nm3

SASDJT4 – Complex of Candidatus Glomeribacter gigasporarum cyclodipeptide synthase (Cglo-CDPS) with Phe-tRNAPhe

UniProt ID: G2JBB2 (2-291) Candidatus Glomeribacter gigasporarum cyclodipeptide synthase

UniProt ID: None (None-None) E. coli Phe-tRNAPhe
Candidatus Glomeribacter gigasporarum cyclodipeptide synthaseE. coli Phe-tRNAPhe experimental SAS data
Candidatus Glomeribacter gigasporarum cyclodipeptide synthase E. coli Phe-tRNAPhe Kratky plot
Sample: Candidatus Glomeribacter gigasporarum cyclodipeptide synthase monomer, 34 kDa Candidatus Glomeribacter gigasporarum protein
E. coli Phe-tRNAPhe monomer, 25 kDa Escherichia coli RNA
Buffer: 10 mM MOPS pH6.7; 200 mM NaCl, 8 mM MgCl2, pH: 6.7
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Oct 2
Structural basis of the interaction between cyclodipeptide synthases and aminoacylated tRNA substrates. RNA 26(11):1589-1602 (2020)
Bourgeois G, Seguin J, Babin M, Gondry M, Mechulam Y, Schmitt E
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 77 nm3

SASDJ35 – Glucose-6-phosphate dehydrogenase dimer

UniProt ID: P11413 (1-515) Glucose-6-phosphate 1-dehydrogenase
Glucose-6-phosphate 1-dehydrogenase experimental SAS data
PYMOL model
Sample: Glucose-6-phosphate 1-dehydrogenase dimer, 119 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 Jul 24
Long-range structural defects by pathogenic mutations in most severe glucose-6-phosphate dehydrogenase deficiency Proceedings of the National Academy of Sciences 118(4) (2021)
Horikoshi N, Hwang S, Gati C, Matsui T, Castillo-Orellana C, Raub A, Garcia A, Jabbarpour F, Batyuk A, Broweleit J, Xiang X, Chiang A, Broweleit R, Vöhringer-Martinez E, Mochly-Rosen D, Wakatsuki S
RgGuinier 3.6 nm
Dmax 12.1 nm
VolumePorod 160 nm3

SASDJ45 – Glucose-6-phosphate dehydrogenase P396L mutant

UniProt ID: P11413 (1-515) Glucose-6-phosphate 1-dehydrogenase P396L
Glucose-6-phosphate 1-dehydrogenase P396L experimental SAS data
CORAL model
Sample: Glucose-6-phosphate 1-dehydrogenase P396L dimer, 119 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 Jul 24
Long-range structural defects by pathogenic mutations in most severe glucose-6-phosphate dehydrogenase deficiency Proceedings of the National Academy of Sciences 118(4) (2021)
Horikoshi N, Hwang S, Gati C, Matsui T, Castillo-Orellana C, Raub A, Garcia A, Jabbarpour F, Batyuk A, Broweleit J, Xiang X, Chiang A, Broweleit R, Vöhringer-Martinez E, Mochly-Rosen D, Wakatsuki S
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 178 nm3

SASDJ75 – apo Archaeoglobus fulgidus Argonaute protein

UniProt ID: O28951 (1-427) Piwi protein AF_1318
Piwi protein AF_1318 experimental SAS data
OTHER model
Sample: Piwi protein AF_1318 dimer, 98 kDa Archaeoglobus fulgidus protein
Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 500 mM NaCl and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
Apo Archaeoglobus fulgidus Argonaute protein dimerises in solution
Elena Manakova
RgGuinier 3.8 nm
Dmax 12.1 nm
VolumePorod 174 nm3

SASDJ85 – Zinc transporter 1 – hZnT1-CTD

UniProt ID: Q9Y6M5 (342-507) Zinc transporter 1
Zinc transporter 1 experimental SAS data
DAMFILT model
Sample: Zinc transporter 1 dimer, 37 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 14
Heterologous Expression and Biochemical Characterization of the Human Zinc Transporter 1 (ZnT1) and Its Soluble C-Terminal Domain Frontiers in Chemistry 9 (2021)
Cotrim C, Jarrott R, Whitten A, Choudhury H, Drew D, Martin J
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 50 nm3

SASDJ95 – E3 ubiquitin-protein ligase BRE1 complexed with E2 ubiquitin conjugating enzyme RAD6

UniProt ID: Q07457 (1-212) E3 ubiquitin-protein ligase BRE1

UniProt ID: P06104 (1-172) Ubiquitin-conjugating enzyme E2 2
E3 ubiquitin-protein ligase BRE1Ubiquitin-conjugating enzyme E2 2 experimental SAS data
SREFLEX model
Sample: E3 ubiquitin-protein ligase BRE1 dimer, 50 kDa Saccharomyces cerevisiae (strain … protein
Ubiquitin-conjugating enzyme E2 2 monomer, 20 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Feb 11
Structural basis for the role of C-terminus acidic tail of Saccharomyces cerevisiae ubiquitin-conjugating enzyme (Rad6) in E3 ligase (Bre1) mediated recognition of histones International Journal of Biological Macromolecules :127717 (2023)
Yadav P, Gupta M, Wazahat R, Islam Z, Tsutakawa S, Kamthan M, Kumar P
RgGuinier 4.1 nm
Dmax 10.4 nm
VolumePorod 105 nm3

SASDJA5 – E2 ubiquitin conjugating enzyme RAD6

UniProt ID: P06104 (1-172) Ubiquitin-conjugating enzyme E2 2
Ubiquitin-conjugating enzyme E2 2 experimental SAS data
SREFLEX model
Sample: Ubiquitin-conjugating enzyme E2 2 monomer, 20 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Feb 11
Structural basis for the role of C-terminus acidic tail of Saccharomyces cerevisiae ubiquitin-conjugating enzyme (Rad6) in E3 ligase (Bre1) mediated recognition of histones International Journal of Biological Macromolecules :127717 (2023)
Yadav P, Gupta M, Wazahat R, Islam Z, Tsutakawa S, Kamthan M, Kumar P
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 31 nm3