SASBDB entries for UniProt ID:

SASDK24 – Signal recognition particle SRP9/14 heterodimer from Plasmodium falciparum

UniProt ID: F2X1X5 (1-103) Signal recognition particle 9

UniProt ID: F2X1X6 (1-104) Signal recognition particle 14
Signal recognition particle 9Signal recognition particle 14 experimental SAS data
DAMMIN model
Sample: Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein
Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 22
Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation. Commun Biol 4(1):600 (2021)
Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I
RgGuinier 2.1 nm
Dmax 7.2 nm
VolumePorod 47 nm3

SASDK54 – Signal recognition particle SRP9/14 heterodimer in complex with full length SRP Alu RNA from Plasmodium falciparum

UniProt ID: F2X1X5 (1-103) Signal recognition particle 9

UniProt ID: F2X1X6 (1-104) Signal recognition particle 14

UniProt ID: None (None-None) Full-length SRP Alu RNA
Signal recognition particle 9Signal recognition particle 14Full-length SRP Alu RNA experimental SAS data
MONSA model
Sample: Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein
Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein
Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 22
Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation. Commun Biol 4(1):600 (2021)
Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 120 nm3

SASDK64 – Signal recognition particle SRP9/14 heterodimer in complex with the 5' domain of SRP Alu RNA from Plasmodium falciparum

UniProt ID: F2X1X5 (1-103) Signal recognition particle 9

UniProt ID: F2X1X6 (1-104) Signal recognition particle 14

UniProt ID: None (None-None) SRP Alu RNA 5' domain
Signal recognition particle 9Signal recognition particle 14SRP Alu RNA 5' domain experimental SAS data
MONSA model
Sample: Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein
Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein
SRP Alu RNA 5' domain monomer, 24 kDa Plasmodium falciparum RNA
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 22
Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation. Commun Biol 4(1):600 (2021)
Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I
RgGuinier 3.2 nm
Dmax 11.9 nm
VolumePorod 77 nm3

SASDK74 – Streptococcus agalactiae transcription factor BusR - RCK_C domain

UniProt ID: Q8E533 (134-213) Transcriptional repressor BusR RCK_C domain
Transcriptional repressor BusR RCK_C domain experimental SAS data
OTHER model
Sample: Transcriptional repressor BusR RCK_C domain dimer, 22 kDa Streptococcus agalactiae serotype … protein
Buffer: 100 mM NaCl, 30mM Hepes, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G
RgGuinier 1.9 nm
Dmax 6.4 nm
VolumePorod 44 nm3

SASDK84 – Streptococcus agalactiae transcription factor BusR

UniProt ID: Q8E533 (1-213) Transcriptional repressor BusR
Transcriptional repressor BusR experimental SAS data
Transcriptional repressor BusR Kratky plot
Sample: Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein
Buffer: 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G
RgGuinier 4.4 nm
Dmax 13.9 nm
VolumePorod 168 nm3

SASDK94 – Streptococcus agalactiae transcription factor BusR dsDNA complex

UniProt ID: Q8E533 (1-213) Transcriptional repressor BusR

UniProt ID: None (None-None) BusR Recognition sequence
Transcriptional repressor BusRBusR Recognition sequence experimental SAS data
OTHER model
Sample: Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein
BusR Recognition sequence monomer, 28 kDa synthetic construct DNA
Buffer: 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G
RgGuinier 4.3 nm
Dmax 14.2 nm
VolumePorod 210 nm3

SASDKA4 – Complement factor P, properdin (FP) dimer

UniProt ID: P27918 (28-469) Properdin (dimer)
Properdin (dimer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (dimer) dimer, 110 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR
RgGuinier 8.1 nm
Dmax 24.0 nm

SASDKB4 – Complement factor P, properdin (FP) trimer

UniProt ID: P27918 (28-469) Properdin (trimer)
Properdin (trimer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (trimer) trimer, 165 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR
RgGuinier 10.2 nm
Dmax 27.0 nm

SASDKC4 – Complement factor P, properdin (FP) tetramer

UniProt ID: P27918 (28-469) Properdin (tetramer)
Properdin (tetramer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (tetramer) tetramer, 220 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR
RgGuinier 13.1 nm
Dmax 36.0 nm

SASDKD4 – In vitro DNA protection during starvation protein (Dps)/DNA co-crystallization

UniProt ID: P0ABT2 (None-None) DNA protection during starvation protein
DNA protection during starvation protein experimental SAS data
DNA protection during starvation protein Kratky plot
Sample: DNA protection during starvation protein dodecamer, 224 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 27
Protective Dps-DNA co-crystallization in stressed cells: an in vitro structural study by small-angle X-ray scattering and cryo-electron tomography. FEBS Lett 593(12):1360-1371 (2019)
Dadinova LA, Chesnokov YM, Kamyshinsky RA, Orlov IA, Petoukhov MV, Mozhaev AA, Soshinskaya EY, Lazarev VN, Manuvera VA, Orekhov AS, Vasiliev AL, Shtykova EV