SASBDB entries for UniProt ID:

SASDMH8 – Splicing factor, proline- and glutamine-rich-QM-TM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM-TM / NONO(52-312) heterodimer

UniProt ID: P23246 (276-598) Splicing factor, proline- and glutamine-rich

UniProt ID: Q15233 (53-312) Non-POU domain-containing octamer-binding protein
Splicing factor, proline- and glutamine-richNon-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 8
Structural basis of the zinc-induced cytoplasmic aggregation of the RNA-binding protein SFPQ Nucleic Acids Research 48(6):3356-3365 (2020)
Huang J, Ringuet M, Whitten A, Caria S, Lim Y, Badhan R, Anggono V, Lee M
RgGuinier 3.6 nm
Dmax 16.5 nm
VolumePorod 80 nm3

SASDMJ8 – Sulfite reductase dodecamer

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 11.0 nm
Dmax 32.5 nm
VolumePorod 1720 nm3

SASDMK8 – Sulfite reductase flavoprotein octamer

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 8.6 nm
Dmax 26.0 nm
VolumePorod 830 nm3

SASDML8 – Sulfite reductase hemoprotein

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 13
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 2.3 nm
Dmax 7.2 nm
VolumePorod 71 nm3

SASDMM8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 0% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 11.2 nm
Dmax 30.5 nm

SASDMN8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 20% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 11.3 nm
Dmax 29.5 nm

SASDMP8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 41% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 11.4 nm
Dmax 28.5 nm
VolumePorod 417 nm3

SASDMQ8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 86% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 6.8 nm
Dmax 22.0 nm
VolumePorod 460 nm3

SASDMR8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 100% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-590) Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 8.5 nm
Dmax 25.1 nm

SASDMS8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 0% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)

UniProt ID: P38038 (53-599) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3
Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase. Biophys J (2022)
Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
RgGuinier 3.4 nm
Dmax 13.0 nm