SASBDB entries for UniProt ID:

SASDS79 – Long SARS-CoV-2 Non-structural protein 10 (Nsp10: C-terminal truncation variant)

UniProt ID: P0DTD1 (4254-4385) Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392)
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.6 nm
Dmax 5.4 nm
VolumePorod 19 nm3

SASDS89 – Short SARS-CoV-2 Non-structural protein 10 (Nsp10: N- and C-terminal truncation variant)

UniProt ID: P0DTD1 (4263-4385) Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392)
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.5 nm
Dmax 5.1 nm
VolumePorod 16 nm3

SASDS99 – Full length SARS Non-structural protein 10 (Nsp10)

UniProt ID: P0C6X7 (4231-4378) Replicase polyprotein 1ab (Non-structural protein 10)
Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 19 nm3

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10)

UniProt ID: K9N7C7 (4238-4377) Replicase polyprotein 1ab (Non-structural protein 10)
Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 19 nm3

SASDSB9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB
Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.6 nm
VolumePorod 35 nm3

SASDSC9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB
Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.4 nm
VolumePorod 37 nm3

SASDSD9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB
Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 39 nm3

SASDSE9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli in the presence of 1 mM ribose

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB

UniProt ID: None (None-None) Ribose
Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.0 nm
Dmax 8.3 nm
VolumePorod 35 nm3

SASDSF9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli in the presence of 1 mM ribose

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB

UniProt ID: None (None-None) Ribose
Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 36 nm3

SASDSG9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli in the presence of 1 mM ribose

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB

UniProt ID: None (None-None) Ribose
Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 39 nm3