SASBDB entries for UniProt ID:

SASDSX8 – Solution scattering studies on 20 kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis (ISPC-12)

UniProt ID: Q7AL75 (2-182) 20 kDa accessory protein
20 kDa accessory protein experimental SAS data
GASBOR model
Sample: 20 kDa accessory protein dimer, 42 kDa Bacillus thuringiensis serovar … protein
Buffer: 10 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2023 Aug 15
20-kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis ISPC-12: Purification, characterization, solution scattering and structural analysis International Journal of Biological Macromolecules :127985 (2023)
Kinkar O, Singh R, Prashar A, Kumar A, Hire R, Makde R
RgGuinier 3.0 nm
Dmax 8.6 nm
VolumePorod 67 nm3

SASDSY8 – S9C peptidase from Geobacillus stearothermophilus

UniProt ID: A0A150M835 (17-670) Acylamino-acid-releasing enzyme (I277L, V491A)
Acylamino-acid-releasing enzyme (I277L, V491A) experimental SAS data
GASBOR model
Sample: Acylamino-acid-releasing enzyme (I277L, V491A) tetramer, 308 kDa Geobacillus stearothermophilus protein
Buffer: 10 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2023 Aug 11
Crystal structure and solution scattering of Geobacillus stearothermophilus S9 peptidase reveal structural adaptations for carboxypeptidase activity. FEBS Lett (2024)
Chandravanshi K, Singh R, Bhange GN, Kumar A, Yadav P, Kumar A, Makde RD
RgGuinier 5.3 nm
Dmax 13.0 nm
VolumePorod 424 nm3

SASDSZ8 – The DNA binding domain (DBD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

UniProt ID: P20823 (83-279) Hepatocyte nuclear factor 1-alpha
Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 1 mM TCEP,, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 25
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 2.7 nm
Dmax 10.2 nm
VolumePorod 22 nm3

SASDS29 – The DNA binding and transactivation domains (DBD-TAD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

UniProt ID: P20823 (83-631) Hepatocyte nuclear factor 1-alpha
Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 62 kDa Homo sapiens protein
Buffer: 4 mM Tris, 100 mM NaCl, 1 mM TCEP,, pH: 8.5
Experiment: SAXS data collected at CoSAXS, MAX IV on 2022 Apr 2
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 109 nm3

SASDS69 – Full length SARS-CoV-2 Non-structural protein 10 (Nsp10)

UniProt ID: P0DTD1 (4254-4392) Replicase polyprotein 1ab (Non-structural protein 10)
Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 21 nm3

SASDS79 – Long SARS-CoV-2 Non-structural protein 10 (Nsp10: C-terminal truncation variant)

UniProt ID: P0DTD1 (4254-4385) Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392)
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.6 nm
Dmax 5.4 nm
VolumePorod 19 nm3

SASDS89 – Short SARS-CoV-2 Non-structural protein 10 (Nsp10: N- and C-terminal truncation variant)

UniProt ID: P0DTD1 (4263-4385) Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392)
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.5 nm
Dmax 5.1 nm
VolumePorod 16 nm3

SASDS99 – Full length SARS Non-structural protein 10 (Nsp10)

UniProt ID: P0C6X7 (4231-4378) Replicase polyprotein 1ab (Non-structural protein 10)
Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 19 nm3

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10)

UniProt ID: K9N7C7 (4238-4377) Replicase polyprotein 1ab (Non-structural protein 10)
Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 19 nm3

SASDSB9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB
Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.1 nm
Dmax 8.6 nm
VolumePorod 35 nm3