SASBDB entries for UniProt ID:

SASDSL8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 7.51 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4
Adhesion G-protein coupled receptor G4 experimental SAS data
EOM/RANCH model
Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21
The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces. J Biol Chem :105356 (2023)
Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N
RgGuinier 2.9 nm
Dmax 9.6 nm
VolumePorod 77 nm3

SASDSM8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 9.15 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4
Adhesion G-protein coupled receptor G4 experimental SAS data
EOM/RANCH model
Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21
The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces. J Biol Chem :105356 (2023)
Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N
RgGuinier 2.9 nm
Dmax 10.8 nm
VolumePorod 78 nm3

SASDSU8 – Threonine aldolase 1 tetramer at neutral pH

UniProt ID: Q6XPS7 (41-400) L-threonine aldolase
L-threonine aldolase experimental SAS data
PHENIX model
Sample: L-threonine aldolase tetramer, 165 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 14
One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse. Nat Commun 15(1):3199 (2024)
Malatesta M, Fornasier E, Di Salvo ML, Tramonti A, Zangelmi E, Peracchi A, Secchi A, Polverini E, Giachin G, Battistutta R, Contestabile R, Percudani R
RgGuinier 3.7 nm
Dmax 10.6 nm
VolumePorod 209 nm3

SASDSV8 – Tyrosine-protein kinase Src (c-Src WT)

UniProt ID: P12931 (84-536) Proto-oncogene tyrosine-protein kinase Src
Proto-oncogene tyrosine-protein kinase Src experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Proto-oncogene tyrosine-protein kinase Src monomer, 52 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1% glycerol and 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 8
An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-Src function Nature Communications 14(1) (2023)
Cuesta-Hernández H, Contreras J, Soriano-Maldonado P, Sánchez-Wandelmer J, Yeung W, Martín-Hurtado A, Muñoz I, Kannan N, Llimargas M, Muñoz J, Plaza-Menacho I
RgGuinier 2.6 nm
Dmax 8.9 nm
VolumePorod 67 nm3

SASDSW8 – Apo form of vaccinia virus polymerase holoenzyme E9-A20-D4

UniProt ID: P20509 (2-1006) DNA polymerase

UniProt ID: P20995 (1-426) DNA polymerase processivity factor component OPG148

UniProt ID: P20536 (1-218) Uracil-DNA glycosylase
DNA polymeraseDNA polymerase processivity factor component OPG148Uracil-DNA glycosylase experimental SAS data
DNA polymerase DNA polymerase processivity factor component OPG148 Uracil-DNA glycosylase Kratky plot
Sample: DNA polymerase monomer, 117 kDa Vaccinia virus (strain … protein
DNA polymerase processivity factor component OPG148 monomer, 49 kDa Vaccinia virus (strain … protein
Uracil-DNA glycosylase monomer, 27 kDa Vaccinia virus (strain … protein
Buffer: 200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 25
Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLoS Pathog 20(5):e1011652 (2024)
Burmeister WP, Boutin L, Balestra AC, Gröger H, Ballandras-Colas A, Hutin S, Kraft C, Grimm C, Böttcher B, Fischer U, Tarbouriech N, Iseni F
RgGuinier 5.0 nm
Dmax 18.0 nm
VolumePorod 350 nm3

SASDSX8 – Solution scattering studies on 20 kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis (ISPC-12)

UniProt ID: Q7AL75 (2-182) 20 kDa accessory protein
20 kDa accessory protein experimental SAS data
GASBOR model
Sample: 20 kDa accessory protein dimer, 42 kDa Bacillus thuringiensis serovar … protein
Buffer: 10 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2023 Aug 15
20-kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis ISPC-12: Purification, characterization, solution scattering and structural analysis International Journal of Biological Macromolecules :127985 (2023)
Kinkar O, Singh R, Prashar A, Kumar A, Hire R, Makde R
RgGuinier 3.0 nm
Dmax 8.6 nm
VolumePorod 67 nm3

SASDSY8 – S9C peptidase from Geobacillus stearothermophilus

UniProt ID: A0A150M835 (17-670) Acylamino-acid-releasing enzyme (I277L, V491A)
Acylamino-acid-releasing enzyme (I277L, V491A) experimental SAS data
GASBOR model
Sample: Acylamino-acid-releasing enzyme (I277L, V491A) tetramer, 308 kDa Geobacillus stearothermophilus protein
Buffer: 10 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2023 Aug 11
Crystal structure and solution scattering of Geobacillus stearothermophilus S9 peptidase reveal structural adaptations for carboxypeptidase activity. FEBS Lett (2024)
Chandravanshi K, Singh R, Bhange GN, Kumar A, Yadav P, Kumar A, Makde RD
RgGuinier 5.3 nm
Dmax 13.0 nm
VolumePorod 424 nm3

SASDSZ8 – The DNA binding domain (DBD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

UniProt ID: P20823 (83-279) Hepatocyte nuclear factor 1-alpha
Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 1 mM TCEP,, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 25
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 2.7 nm
Dmax 10.2 nm
VolumePorod 22 nm3

SASDS29 – The DNA binding and transactivation domains (DBD-TAD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

UniProt ID: P20823 (83-631) Hepatocyte nuclear factor 1-alpha
Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 62 kDa Homo sapiens protein
Buffer: 4 mM Tris, 100 mM NaCl, 1 mM TCEP,, pH: 8.5
Experiment: SAXS data collected at CoSAXS, MAX IV on 2022 Apr 2
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 109 nm3

SASDS69 – Full length SARS-CoV-2 Non-structural protein 10 (Nsp10)

UniProt ID: P0DTD1 (4254-4392) Replicase polyprotein 1ab (Non-structural protein 10)
Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 21 nm3