SASBDB entries for UniProt ID:

SASDSH9 – SAXS data of Clostridium perfringens enterotoxin

UniProt ID: P01558 (1-319) Heat-labile enterotoxin B chain
Heat-labile enterotoxin B chain experimental SAS data
GASBOR model
Sample: Heat-labile enterotoxin B chain monomer, 36 kDa Clostridium perfringens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18
Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A
RgGuinier 2.7 nm
Dmax 10.2 nm
VolumePorod 46 nm3

SASDSJ9 – SAXS data of trypsinized Clostridium perfringens enterotoxin

UniProt ID: P01558 (26-319) Heat-labile enterotoxin B chain
Heat-labile enterotoxin B chain experimental SAS data
GASBOR model
Sample: Heat-labile enterotoxin B chain monomer, 33 kDa Clostridium perfringens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 18
Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin Toxins 15(11):637 (2023)
Ogbu C, Kapoor S, Vecchio A
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 40 nm3

SASDSM9 – Hen egg white lysozyme in 1 mol% ethylammonium nitrate

UniProt ID: P00698 (19-147) Lysozyme C
Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments Advances in Colloid and Interface Science :103242 (2024)
Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 20 nm3

SASDSN9 – Buffer over-subtracted hen egg white lysozyme in 1 mol% ethylammonium nitrate

UniProt ID: P00698 (19-147) Lysozyme C
Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments Advances in Colloid and Interface Science :103242 (2024)
Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 28 nm3

SASDSP9 – Buffer under-substracted hen egg white lysozyme in 1mol% ethylammonium nitrate

UniProt ID: P00698 (19-147) Lysozyme C
Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments Advances in Colloid and Interface Science :103242 (2024)
Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 23 nm3

SASDSQ9 – NanoLuc luciferase

UniProt ID: None (None-None) Oplophorus-luciferin 2-monooxygenase catalytic subunit
Oplophorus-luciferin 2-monooxygenase catalytic subunit experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Oplophorus-luciferin 2-monooxygenase catalytic subunit monomer, 20 kDa Oplophorus gracilirostris protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2021 May 27
Illuminating the mechanism and allosteric behavior of NanoLuc luciferase. Nat Commun 14(1):7864 (2023)
Nemergut M, Pluskal D, Horackova J, Sustrova T, Tulis J, Barta T, Baatallah R, Gagnot G, Novakova V, Majerova M, Sedlackova K, Marques SM, Toul M, Damborsky J, Prokop Z, Bednar D, Janin YL, Marek M
RgGuinier 1.8 nm
Dmax 5.9 nm
VolumePorod 38 nm3

SASDT82 – Halophilic Lrp transcription factor

UniProt ID: I3R177 (1-152) AsnC family transcriptional regulator
AsnC family transcriptional regulator experimental SAS data
PHENIX model
Sample: AsnC family transcriptional regulator dimer, 36 kDa Haloferax mediterranei (strain … protein
Buffer: 50 mM Tris, 1.5 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 12
Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization. Int J Biol Macromol :129541 (2024)
Matarredona L, García-Bonete MJ, Guío J, Camacho M, Fillat MF, Esclapez J, Bonete MJ
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 70 nm3

SASDT92 – Prion-like domain of 29 kDa chloroplastic ribonucleoprotein measured at at 298 K from Arabidopsis thaliana (Mouse-ear cress)

UniProt ID: Q43349 (176-254) 29 kDa ribonucleoprotein, chloroplastic
29 kDa ribonucleoprotein, chloroplastic experimental SAS data
29 kDa ribonucleoprotein, chloroplastic Kratky plot
Sample: 29 kDa ribonucleoprotein, chloroplastic monomer, 8 kDa Arabidopsis thaliana protein
Buffer: 20 mM sodium phosphate, 30 mM NaCl, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2021 Oct 19
A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis. Plant Cell (2024)
Legen J, Lenzen B, Kachariya N, Feltgen S, Gao Y, Mergenthal S, Weber W, Klotzsch E, Zoschke R, Sattler M, Schmitz-Linneweber C
RgGuinier 2.1 nm
Dmax 9.2 nm
VolumePorod 19 nm3

SASDTA2 – Prion-like domain of 29 kDa chloroplastic ribonucleoprotein measured at at 277 K from Arabidopsis thaliana (Mouse-ear cress)

UniProt ID: Q43349 (176-254) 29 kDa ribonucleoprotein, chloroplastic
29 kDa ribonucleoprotein, chloroplastic experimental SAS data
29 kDa ribonucleoprotein, chloroplastic Kratky plot
Sample: 29 kDa ribonucleoprotein, chloroplastic monomer, 8 kDa Arabidopsis thaliana protein
Buffer: 20 mM sodium phosphate, 30 mM NaCl, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2021 Oct 19
A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis. Plant Cell (2024)
Legen J, Lenzen B, Kachariya N, Feltgen S, Gao Y, Mergenthal S, Weber W, Klotzsch E, Zoschke R, Sattler M, Schmitz-Linneweber C
RgGuinier 2.4 nm
Dmax 11.6 nm
VolumePorod 25 nm3

SASDTB2 – WAXS data of poly-histidine tagged Ribose binding protein (16 mg/mL) from Escherichia coli

UniProt ID: P02925 (25-296) Ribose import binding protein RbsB
Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
SAXS/WAXS data of conformationally flexible ribose binding protein Data in Brief :109932 (2023)
Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
RgGuinier 2.5 nm
Dmax 12.0 nm
VolumePorod 36 nm3