SASBDB entries for UniProt ID:

SASDUT6 – Nuclear fusion protein BIK1 in high salt buffer

UniProt ID: P11709 (2-440) Nuclear fusion protein BIK1
Nuclear fusion protein BIK1 experimental SAS data
DAMFILT model
Sample: Nuclear fusion protein BIK1 dimer, 106 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jul 13
Phase separation of a microtubule plus-end tracking protein into a fluid fractal network (2024)
Czub M, Uliana F, Grubić T, Padeste C, Rosowski K, Dufresne E, Menzel A, Vakonakis I, Gasser U, Steinmetz M
RgGuinier 8.8 nm
Dmax 28.0 nm
VolumePorod 467 nm3

SASDUU6 – Nuclear fusion protein BIK1 coiled-coil in a high salt buffer

UniProt ID: P11709 (182-389) Nuclear fusion protein BIK1
Nuclear fusion protein BIK1 experimental SAS data
DAMFILT model
Sample: Nuclear fusion protein BIK1 dimer, 55 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jul 13
Phase separation of a microtubule plus-end tracking protein into a fluid fractal network (2024)
Czub M, Uliana F, Grubić T, Padeste C, Rosowski K, Dufresne E, Menzel A, Vakonakis I, Gasser U, Steinmetz M
RgGuinier 8.0 nm
Dmax 27.0 nm
VolumePorod 282 nm3

SASDUV6 – Nuclear fusion protein BIK1 ΔQQFF in a high salt buffer

UniProt ID: P11709 (2-436) Nuclear fusion protein BIK1
Nuclear fusion protein BIK1 experimental SAS data
DAMFILT model
Sample: Nuclear fusion protein BIK1 dimer, 105 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jul 13
Phase separation of a microtubule plus-end tracking protein into a fluid fractal network (2024)
Czub M, Uliana F, Grubić T, Padeste C, Rosowski K, Dufresne E, Menzel A, Vakonakis I, Gasser U, Steinmetz M
RgGuinier 9.4 nm
Dmax 29.4 nm
VolumePorod 632 nm3

SASDUW6 – Alarmin release inhibitor (HpARI2), full-length

UniProt ID: A0A3P7XL18 (63-297) Alarmin release inhibitor (Δ1-62)
Alarmin release inhibitor (Δ1-62) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.6 nm
Dmax 9.9 nm
VolumePorod 31 nm3

SASDUX6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3

UniProt ID: A0A3P7XL18 (126-297) Alarmin release inhibitor (Δ1-125; N175Q, N190Q)
Alarmin release inhibitor (Δ1-125; N175Q, N190Q) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 28 nm3

SASDUY6 – Alarmin release inhibitor (HpARI2), full length, bound to Interleukin-33

UniProt ID: A0A3P7XL18 (63-297) Alarmin release inhibitor (Δ1-62)

UniProt ID: Q8BVZ5 (109-266) Interleukin-33 (L179V)
Alarmin release inhibitor (Δ1-62)Interleukin-33 (L179V) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein
Interleukin-33 (L179V) monomer, 18 kDa Mus musculus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.7 nm
Dmax 9.7 nm
VolumePorod 62 nm3

SASDUZ6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3 bound to Interleukin-33

UniProt ID: Q8BVZ5 (109-266) Interleukin-33 (L179V)

UniProt ID: A0A3P7XL18 (126-297) Alarmin release inhibitor (Δ1-125; N175Q, N190Q)
Interleukin-33 (L179V)Alarmin release inhibitor (Δ1-125; N175Q, N190Q) experimental SAS data
REFMAC model
Sample: Interleukin-33 (L179V) monomer, 18 kDa Mus musculus protein
Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.5 nm
Dmax 8.3 nm
VolumePorod 58 nm3

SASDU27 – The DNA binding domain and intrinsically disordered region of NAC domain-containing protein 13 (ANAC013; amino acids 1-274)

UniProt ID: F4IED2 (1-274) NAC domain-containing protein 13
NAC domain-containing protein 13 experimental SAS data
NAC domain-containing protein 13 Kratky plot
Sample: NAC domain-containing protein 13 dimer, 63 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Allovalent scavenging of activation domains in the transcription factor ANAC013 gears transcriptional regulation. Nucleic Acids Res 53(4) (2025)
Delaforge E, Due AD, Theisen FF, Morffy N, O'Shea C, Blackledge M, Strader LC, Skriver K, Kragelund BB
RgGuinier 3.8 nm
Dmax 17.0 nm
VolumePorod 118 nm3

SASDU37 – The intrinsically disordered region of NAC domain-containing protein 13 (ANAC013; amino acids 161-274)

UniProt ID: F4IED2 (161-274) NAC domain-containing protein 13
NAC domain-containing protein 13 experimental SAS data
NAC domain-containing protein 13 Kratky plot
Sample: NAC domain-containing protein 13 monomer, 13 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Allovalent scavenging of activation domains in the transcription factor ANAC013 gears transcriptional regulation. Nucleic Acids Res 53(4) (2025)
Delaforge E, Due AD, Theisen FF, Morffy N, O'Shea C, Blackledge M, Strader LC, Skriver K, Kragelund BB
RgGuinier 3.6 nm
Dmax 16.0 nm
VolumePorod 36 nm3

SASDU47 – Non-homologous end-joining factor 1 - XLF

UniProt ID: Q9H9Q4 (1-299) Non-homologous end-joining factor 1
Non-homologous end-joining factor 1 experimental SAS data
Non-homologous end-joining factor 1 Kratky plot
Sample: Non-homologous end-joining factor 1 dimer, 67 kDa Homo sapiens protein
Buffer: 20 mM Bis-tris, 150 mM KCl, 1 mM EDTA, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Mar 13
Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro. Nat Struct Mol Biol (2024)
Vu DD, Bonucci A, Brenière M, Cisneros-Aguirre M, Pelupessy P, Wang Z, Carlier L, Bouvignies G, Cortes P, Aggarwal AK, Blackledge M, Gueroui Z, Belle V, Stark JM, Modesti M, Ferrage F
RgGuinier 3.6 nm
Dmax 12.1 nm
VolumePorod 123 nm3