SASBDB entries for UniProt ID:

SASDLK8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.330)

UniProt ID: P0DN75 (2-132) Iron-sulfur cluster assembly 1 homolog, mitochondrial
Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.0 nm

SASDMJ7 – ESX-1 secretion-associated protein EspB small construct bound to ESX-1 secretion-associated protein EspK — EspBS-K complex

UniProt ID: P9WJC1 (484-729) ESX-1 secretion-associated protein EspK

UniProt ID: P9WJD9 (7-278) ESX-1 secretion-associated protein EspB
ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspK ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 113 nm3

SASDP62 – dark-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

UniProt ID: P74102 (2-317) Orange carotenoid-binding protein
Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 58 nm3

SASDPS2 – apo-Thermus thermophilus argonaute protein at 10 °C

UniProt ID: Q746M7 (1-685) Piwi domain-containing protein
Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 122 nm3

SASDPR6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2
NucleoproteinStem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 51 nm3

SASDRF5 – A ssr1698 Dri1 hemoprotein, H21A variant

UniProt ID: P73129 (1-96) Ssr1698 protein (H21A)
Ssr1698 protein (H21A) experimental SAS data
Sample: Ssr1698 protein (H21A) monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 10
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.7 nm
VolumePorod 12 nm3

SASDRH6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to a doubly phosphorylated Dok1 peptide

UniProt ID: P20936 (174-1047) Ras GTPase-activating protein 1

UniProt ID: Q99704 (291-322) Docking protein 1
Ras GTPase-activating protein 1Docking protein 1 experimental SAS data
Ras GTPase-activating protein 1 Docking protein 1 Kratky plot
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Docking protein 1 monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 4.0 nm
Dmax 14.6 nm
VolumePorod 196 nm3

SASDSX7 – Human phosphorylated CaMKK2 bound to 14-3-3 gamma

UniProt ID: P61981 (1-234) 14-3-3 protein gamma

UniProt ID: Q96RR4 (93-517) Calcium/calmodulin-dependent protein kinase kinase 2
14-3-3 protein gammaCalcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
ALLOSMOD model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 157 nm3

SASDSR9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 38.2 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
RgGuinier 3.3 nm
Dmax 15.5 nm
VolumePorod 56 nm3

SASDT83 – Teneurin-3 A0B0 isoform in 5 mM EDTA - 0.52 mg/mL

UniProt ID: Q9WTS6-2 (343-2699) Isoform A0B0 of Teneurin-3
Isoform A0B0 of Teneurin-3 experimental SAS data
Isoform A0B0 of Teneurin-3 Kratky plot
Sample: Isoform A0B0 of Teneurin-3 dimer, 526 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM EDTA, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10
Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition Nature Communications 15(1) (2024)
Gogou C, Beugelink J, Frias C, Kresik L, Jaroszynska N, Drescher U, Janssen B, Hindges R, Meijer D
RgGuinier 8.6 nm
Dmax 33.0 nm
VolumePorod 990 nm3